Evaluation of the automated Becton Dickinson MAX real-time PCR platform for detection of Pneumocystis jirovecii

被引：13
|
作者：
Chien, Jung-Yien [1 ]
Liu, Chia-Jung [1 ]
Chuang, Pei-Chien [2 ]
Lee, Tai-Fen [2 ]
Huang, Yu-Tsung [3 ,4 ]
Liao, Chun-Hsing [3 ]
Hung, Chien-Ching [1 ]
Sheng, Wan-Huei [1 ]
Yu, Chong-Jen [1 ]
Hsueh, Po-Ren [1 ,2 ]
机构：
[1] Natl Taiwan Univ, Coll Med, Dept Internal Med, Natl Taiwan Univ Hosp, Taipei, Taiwan
[2] Natl Taiwan Univ, Coll Med, Dept Lab Med, Natl Taiwan Univ Hosp, Taipei, Taiwan
[3] Far Eastern Mem Hosp, Dept Internal Med, New Taipei, Taiwan
[4] Natl Taiwan Univ, Grad Inst Clin Lab Sci & Med Biotechnol, Taipei, Taiwan
来源：
FUTURE MICROBIOLOGY | 2017年 / 12卷 / 01期
关键词：
major surface glycoprotein; MSG; mtLSU PCR; nested mitochondrial large subunit PCR; PCR; Pneumocystis jirovecii; P. jirovecii pneumonia; real-time PCR; CLINICAL-SIGNIFICANCE; MOLECULAR DIAGNOSIS; PNEUMONIA; ASSAY; COLONIZATION; CARINII; SPECIMENS; DNA;
D O I：
10.2217/fmb-2016-0115
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Aim: We evaluated the performance of the automated quantitative BD MAX (Becton Dickinson) real-time PCR platform for detecting Pneumocystis jirovecii. Materials & methods: A total of 34 retrospective and 137 prospective samples were included. Results: Retrospectively, all (100%) positive samples were correctly detected by this platform compared with a nested PCR. Among prospective samples, the overall sensitivity, specificity, positive likelihood ratio and negative likelihood ratio were 92.6%, 94.5%, 17.0 and 0.1, respectively. All bronchoalveolar lavage fluid (BALF)/bronchial washing samples were correctly identified by this platform. Samples from patients with colonization had significantly higher median amplification cycle threshold values than patients with P. jirovecii pneumonia. Conclusion: The quantitative BD MAX real-time PCR is a rapid and highly sensitive modality for detecting P. jirovecii, especially in samples from bronchoalveolar lavage fluid/bronchial washing fluid.
引用
收藏
页码：29 / 37
页数：9
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