A fluorogenic assay for screening Sirt6 modulators

被引：39
|
作者：
Hu, Jing [1 ]
He, Bin [1 ]
Bhargava, Shiva [1 ]
Lin, Hening [1 ]
机构：
[1] Cornell Univ, Baker Lab, Dept Chem & Chem Biol, Ithaca, NY 14853 USA
来源：
ORGANIC & BIOMOLECULAR CHEMISTRY | 2013年 / 11卷 / 32期
关键词：
SMALL-MOLECULE ACTIVATORS; SIRTUINS; INHIBITION; IDENTIFICATION; DEACETYLASES; MECHANISM; INSIGHTS; MODELS;
D O I：
10.1039/c3ob41138a
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
A fluorogenic high-throughput assay suitable for screening Sirt6 modulators is developed based on the recently discovered efficient activity of Sirt6 to hydrolyze myristoyl lysine. Sirt6 modulators will be useful in investigating the function of Sirt6 and protein lysine fatty acylation.
引用
收藏
页码：5213 / 5216
页数：4
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