Sialic acid sulfation is induced by the antibiotic treatment in mammalian cells

被引：5
|
作者：
Ertunc, Nursah [1 ,2 ]
Sato, Chihiro [1 ,2 ,3 ]
Kitajima, Ken [1 ,2 ,3 ]
机构：
[1] Nagoya Univ, Biosci & Biotechnol Ctr, Nagoya, Aichi, Japan
[2] Nagoya Univ, Grad Sch Bioagr Sci, Nagoya, Aichi, Japan
[3] Tokai Natl Higher Educ & Res Syst, Inst Glycocore Res, Nagoya, Aichi, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2020年 / 84卷 / 11期
关键词：
Sialic acid; sulfated sialic acid; antibiotic G418; inducible sulfation; mammal; SEA-URCHIN SPERM; POLYSIALIC ACID; SULFOTRANSFERASES; AMINOGLYCOSIDE; IDENTIFICATION; GLYCOPROTEINS; GANGLIOSIDES; EXPRESSION; CHAIN; EGGS;
D O I：
10.1080/09168451.2020.1792763
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Sialic acids (Sias) are an outermost-situated sugar of glycoproteins and glycolipids to play important roles in various biological phenomena. They are often modified by additional substituents, such asO-acetyl group, to display more than 50 different structures in nature. Of those modified Sia, nothing is known about the occurrence and biological functions of sulfated Sias (SiaSs) in mammals. To elucidate the significance of sialic acid sulfation, we investigated various mammalian-cultured cell lines for the expression of SiaS using the specific antibody 3G9. First, SiaS is expressed in a cell line-dependent and a cell density-dependent manner. Second, in CHO cells, the expression of SiaS is reversibly induced by treatment with the antibiotic G418. Taken together, the expression of SiaS is changed by intrinsic and extrinsic factors in mammalian cells. This is the first demonstration of regulated expression of SiaS.
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收藏
页码：2311 / 2318
页数：8
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