Analysis of aged gelatin by AFlFFF-MALS: Identification of high molar mass components and their influence on solubility

被引：15

作者：

Rbii, Khalid ^{[1
,2
]}

Violleau, Frederic ^{[1
]}

Guedj, Sylviane ^{[2
]}

Surel, Olivier ^{[1
]}

机构：

[1] Univ Toulouse, Ecole Ingn Purpan, Lab Agrophysiol, UPSP DGER 115, F-31076 Toulouse 03, France

[2] Rousselot Isle Sorgue SAS, F-84800 Lisle Sur La Sorgue, France

来源：

FOOD HYDROCOLLOIDS | 2009年 / 23卷 / 03期

关键词：

Asymmetrical flow field-flow fractionation; Multi-angle light scattering; Ageing process; Gelatin; Solubility; Aggregation; FIELD-FLOW FRACTIONATION; MULTIANGLE LIGHT-SCATTERING; CROSS-LINKING; SIZE CHARACTERIZATION; CAPSULES; DISSOLUTION; SEPARATION; CHROMATOGRAPHY; SPECTROSCOPY; FORMALDEHYDE;

D O I：

10.1016/j.foodhyd.2008.08.004

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Gelatin is an important product for several industries and its solubility dramatically influences its functional properties. In order to be able to predict the gelatin behaviour, a new technique for its analysis has been developed with an Asymmetrical Flow Field-Flow Fractionation coupled to a multi-angle light scattering. The AFlFFF-MALS analysis showed the gelatin molar mass ranging from 5 x 104 to 2 X 107 g mol(-1). This technique also permitted to follow aggregation of gelatin samples after process in an oven at 75 degrees C. Between 0 and 4 days, some huge aggregates appeared. Their size and density increased without changing gelatin solubility. From 8 to 30 days, the molar mass and density of these aggregates increased leading to partial gelatin insolubilisation in water. This phenomenon is Supposed to be due to cross-linking of the gelatin macromolecules. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：1024 / 1030

页数：7

共 4 条

[1] Study of gelatin renaturation in aqueous solution by AFlFFF-MALS: Influence of a thermal pre-treatment applied on gelatin
Rbii, Khalid
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[J]. FOOD HYDROCOLLOIDS, 2011, 25 (03) : 511 - 514
[2] Comprehensive analysis of pig feces metabolome by chromatographic techniques coupled to mass spectrometry in high resolution mode: Influence of sample preparation on the identification coverage
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[3] Cell extraction coupled with high performance liquid chromatography-electrospray ionization time-of-flight mass spectrometry analysis for screening and identification of potential bioactive components in Radix Astragali
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[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2008, 36 (06) : 745 - 749
[4] ID-CUBE direct analysis in real time high-resolution mass spectrometry and its capabilities in the identification of phenolic components from the green leaves of Bergenia crassifolia L.
Chernetsova, Elena S.
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[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2012, 26 (11) : 1329 - 1337

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