The Effect and Mechanism of Emodin on the NO Secretion of Human Umbilical Vein Endothelial Cells (HUVECs) Induced by High Glucose

被引:0
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作者
Li, Yajia [1 ,2 ,3 ]
Li, Qiangxiang [4 ,5 ,6 ]
Xie, Chunyan [6 ]
Huang, Yanfei [5 ]
Jia, Limin [5 ]
机构
[1] Cent South Univ, Xiangya Hosp, Dept Dermatol, Changsha, Peoples R China
[2] Cent South Univ, Hunan Engn Res Ctr Skin Hlth & Dis, Changsha, Peoples R China
[3] Cent South Univ, Hunan Key Lab Skin Canc & Psoriasis, Changsha, Hunan, Peoples R China
[4] Cent South Univ, Xiangya Hosp, Natl Clin Res Ctr Geriatr Disorders, Changsha 410008, Hunan, Peoples R China
[5] Natl Clin Res Ctr Geriatr Disorders XIANGYA, Changsha 410008, Hunan, Peoples R China
[6] Nanhua Univ, Loudi Affiliated Hosp, Loudi 417000, Hunan, Peoples R China
关键词
DYSFUNCTION; ADIPONECTIN; METABOLISM; APOPTOSIS; PATHWAY;
D O I
10.1155/2020/4832840
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective. To investigate the effects of emodin on nitric oxide (NO) secretion induced by high glucose in human umbilical vein endothelial cells (HUVECs) through the p-Akt signaling pathway.Methods. Sensitivity of cells to emodin was determined by MTT assay to establish the experimental concentrations; then, HUVECs were treated with high-dose (33.3 mmol/L) glucose (HG), HG + emodin (HG + E), HG + the Akt phosphorylation inhibitor LY294002 (HG + LY), or HG + E + LY. The p-Akt (Ser 473) expression in 48 h was analyzed using Western blot. NO effect on the secretion of HUVECs was analyzed using nitrate reductase assay.Results. The sensitive emodin concentration for HUVECs growth was 10 mol/L (P<0.05). Compared with the HG group, NO secretion was significantly higher in the HG + E group (P<0.05), whereas it was lowest in the HG + LY group (P<0.05). Compared with the HG + LY group, NO secretion was increased in the HG + E + LY group (P<0.05). The p-Akt protein expression was decreased in the HG + LY group when compared to the HG group (P<0.05), while it significantly increased in the HG + E group (P<0.05). Compared with HG + LY group, p-Akt protein expression was significantly higher in the HG + E + LY group (P<0.05).Conclusion. Emodin could improve the NO secretion of HUVECs by high glucose through the p-Akt signaling pathway.
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页数:5
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