Non-destructive imaging of an individual protein

被引：15

作者：

Longchamp, J. -N. ^{[1
]}

Latychevskaia, T. ^{[1
]}

Escher, C. ^{[1
]}

Fink, H. -W. ^{[1
]}

机构：

[1] Univ Zurich, Inst Phys, CH-8057 Zurich, Switzerland

来源：

APPLIED PHYSICS LETTERS | 2012年 / 101卷 / 09期

基金：

瑞士国家科学基金会;

关键词：

X-RAY PULSES; ELECTRON HOLOGRAPHY; BIOLOGICAL SPECIMENS; FERRITIN; MICROSCOPY; RESOLUTION; DIFFRACTION; CELLS;

D O I：

10.1063/1.4748113

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

Imaging a single biomolecule at atomic resolution without averaging over different conformations is the ultimate goal in structural biology. We report recordings of a protein at nanometer resolution obtained from one individual ferritin by means of low-energy electron holography. One single protein could be imaged for an extended period of time without any sign of radiation damage. Since the fragile protein shell encloses a robust iron cluster, the holographic reconstructions could also be cross-validated against transmission electron microscopy images of the very same molecule by imaging its iron core. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4748113]

引用

页数：4

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