Investigation of the role of CD1d-restricted invariant NKT cells in experimental choroidal neovascularization

被引：16

作者：

Hijioka, Kuniaki ^{[1
]}

Sonoda, Koh-Hei ^{[1
]}

Tsutsumi-Miyahara, Chikako ^{[1
]}

Fujimoto, Takeshi ^{[1
]}

Oshima, Yuji ^{[1
]}

Taniguchi, Masaru ^{[2
]}

Ishibashi, Tatsuro ^{[1
]}

机构：

[1] Kyushu Univ, Dept Ophthalmol, Grad Sch Med Sci, Higashi Ku, Fukuoka 8128582, Japan

[2] RIKEN, Res Ctr Allergy & Immunol, Lab Immune Regulat, Kanagawa 2300045, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 374卷 / 01期

关键词：

choroidal neovascularization; age-related macular degeneration; natural killer T cell; vascular endothelial growth factor; innate immunity;

D O I：

10.1016/j.bbrc.2008.06.080

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Choroidal neovascularization (CNV) is directly related to visual loss in age-related macular degeneration and other macular disorders. We have investigated the role of CD1d-restricted invariant natural killer T (NKT) cells in laser-induced experimental CNV. Quantitative real-time PCR detected increased expression of NKT cell-related genes (Va14 and CXCL16) in whole eyes undergoing CNV, indicating local accumulation of NKT cells. We found a significant reduction of CNV and lower concentrations of vascular endothelial growth factor (VEGF) in ocular fluid in two different NKT cell-deficient mice, CD1d knockout (KO) and J alpha 18 KO mice. We also established in vitro co-cultures of retinal pigment epithelial cells and splenic NKT cells, and confirmed NKT cells could produce VEGF in the dish. Moreover, inoculating a-galactosylceramide, the ligand for NKT cells, into the vitreous cavity of C57BL/6 mice promoted CNV. We concluded that NKT cells play an important role in CNV as an inducer of VEGF. (C) 2008 Elsevier Inc. All right reserved.

引用

页码：38 / 43

页数：6

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