Size-exclusion high-performance liquid chromatography of peptides: a review

被引:35
|
作者
Irvine, GB
机构
[1] Ctr. for Peptide and Protein Eng., School of Biology and Biochemistry, Queen's University Belfast
关键词
size-exclusion; HPLC; peptides; peptide purification; peptide analysis; molecular weight estimation;
D O I
10.1016/S0003-2670(97)00322-X
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Gel filtration, now referred to as size exclusion chromatography (SEC), on soft gels has been employed for over 40 years for the separation, desalting and molecular weight estimation of peptides and proteins. Developments in rigid particle technology during the 1970s led to the commercial availability of pre-packed columns giving rapid run times and increased resolution, referred to as size-exclusion high-performance chromatography (SE-HPLC). Initially these packings were more suitable for the separation of proteins than of peptides. Today, supports that operate in the fractionation range <10000 Daltons (Da) are available. In this review, the theoretical basis of SEC is discussed and examples are given of its application to the study of peptides, especially regarding purification, estimation of molecular weight and study of molecular associations. (C) 1997 Elsevier Science B.V.
引用
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页码:387 / 397
页数:11
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