Molecular characterization of artemisia virus A, a new sobemovirus isolated from Artemisia annua

被引:4
|
作者
Ramel, Maria-Elena [1 ]
Gugerli, Paul [1 ]
Besse, Sebastien [2 ]
Simonnet, Xavier [3 ]
Balmelli, Carole [1 ]
机构
[1] Agroscope Changins Wadenswil Res Stn ACW, CH-1260 Nyon, Switzerland
[2] Off Arboriculture & Cultures Maraicheres, Serv Agr, CH-1950 Sion, Switzerland
[3] Mediplant, Ctr Rech Conthey, CH-1964 Conthey, Switzerland
关键词
MOTTLE-VIRUS; SEQUENCE; SOFTWARE; PROTEIN; DNA;
D O I
10.1007/s00705-012-1507-4
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A distinct sobemovirus was isolated from diseased Artemisia annua plants grown in experimental culture plots in Switzerland. Electron microscopy performed on extracts of leaf and root samples of a diseased A. annua plant revealed icosahedral-30 nm viral particles. The complete nucleotide sequence of the viral genome was determined. The single positive-strand RNA of 4138 nt encodes four open reading frames with an organization similar to that described for sobemoviruses. Phylogenetic analysis revealed a close relationship to ryegrass mottle virus. The virus was efficiently acquired by healthy A. annua from contaminated soil samples. "Artemisia virus A" is tentatively proposed as a name for this new candidate member of the unassigned genus Sobemovirus.
引用
收藏
页码:463 / 466
页数:4
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