Effects of supplementing green light to red and blue light on the growth and yield of lettuce in plant factories

Red and blue light-emitting diodes (LEDs) are widely used as light sources in plant factories. Plants grown under red and blue light only produces purplish-gray light environment where plant leaves look purplish-gray. Under that light condition, it makes difficulty to check health status of plants specially insect and disease infected leaves by human naked eye. But the leaf color turns green when inspected under full spectrum of light environment. In this regard, the use of green light to red and blue create a white light environment which is congenial for the grower. Likewise, CO2 assimilation of green light is usually lower compared to red and blue light because of its lower absorptance under low PPFD. But at higher PPFD (>= 500 mu mol m- 2 s- 1), green light shows higher CO2 assimilation rate compared to red and blue light through uniform distribution of full spectrum (red, green and blue) of light into the plant canopy and the lower leaves. In this study, optimal intensity of green light supplementation to the red and blue LEDs was evaluated for better growth, yield, and quality of lettuce. In experiment I and II, green light was added with red and blue light where total photosynthetic photon flux density (PPFD) was increased for the additional green light. In experiment III, 0, 10, 20, 30, 40, 50, 60, 70, 80 mu mol m- 2 s- 1 green (G) light was supplemented to 235 mu mol m- 2 s- 1 red (R) and 59 mu mol m- 2 s- 1 blue (B) light maintaining 294 mu mol m- 2 s- 1 total PPFD. Lettuce plants were cultivated hydroponically in three-step vertical grow beds using half-strength of Enshi nutrient solution. The temperature was maintained at 20 +/- 2 degrees C at day and night and the day length was 16 h. In experiment I and II, the fresh mass of lettuce grown under the combination of R, B and G LEDs was found higher than those cultivated under R and B LEDs only. In experiment III, lettuce plants produced high fresh masses when 30 mu mol m- 2 s- 1 of G light was supplemented with R and B lights maintaining the ratio 211:30:53 (R:G:B). By supplementing excessive G light (>= 50 mu mol m- 2 s- 1) with R and B lights, lettuce shoot fresh mass declined. The various combinations of irradiation of R, G and B LED did not significantly affect the contents of ascorbic acid and mineral nutrients. It is recommended to supplement 30 mu mol m- 2 s- 1 of green light to red and blue light for a higher yield of lettuce. Therefore, the suitable LED (R:G:B) combination would be 72% of red, 10% of green and 18% of blue for lettuce cultivation following recycled hydroponics in plant factories.