Ras and Rap1 activation of PLCε lipase activity

被引:12
|
作者
Edamatsu, Hironori [1 ]
Satoh, Takaya [1 ]
Kataoka, Tohru [1 ]
机构
[1] Kobe Univ, Grad Sch Med, Dept Mol & Cellular Biol, Div Mol Biol, Kobe, Hyogo 657, Japan
关键词
D O I
10.1016/S0076-6879(05)07009-6
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Phosphoinositide-specific phospholipase C (PLC) plays a pivotal role in signal transduction from various receptor molecules on the plasma membrane. PLC epsilon is characterized by possession of two Ras/Rap-associating (RA) domains and a CDC25 homology domain acting as a guanine nucleotide exchange factor for Rapt. Our recent studies using PLC epsilon-deficient mice have suggested that PLC epsilon plays crucial roles in cardiac semilunar valvulogenesis downstream of the EGF receptor, as well as in chemical carcinogen-induced skin tumor development downstream of Ha-Ras. Stimulation of cultured mammalian cells with growth factors induces translocation of PLC epsilon from the cytoplasm to the plasma membrane and to the Golgi apparatus through direct association at its RA domains with the GTP-bound forms of Ras and Rap1, respectively. These results suggest that growth factor stimulation activates PLC epsilon by means of Ras and/or Rapt. However, growth factor-induced activation of the PLC epsilon lipase activity cannot be measured accurately because of simultaneous activation of PLC gamma through receptor-dependent phosphorylation. In this article, we introduce two methods to assay Ras- or Rap1-dependent activation of PLC epsilon lipase activity, with special emphasis on the use of cells expressing a mutant platelet-derived growth factor receptor lacking the PLC gamma-binding sites.
引用
收藏
页码:99 / 107
页数:9
相关论文
共 50 条
  • [1] Specific Features of Transient Ras and Sustained Rap1 Activation
    Ozaki, Yu-ichi
    Uda, Shinsuke
    Kuroda, Shinya
    [J]. SYSTEMS BIOLOGY: THE CHALLENGE OF COMPLEXITY, 2009, : 121 - +
  • [2] CalDAG-GEFIII activation of Ras, R-Ras, and Rap1
    Yamashita, S
    Mochizuki, N
    Ohba, Y
    Tobiume, M
    Okada, Y
    Sawa, H
    Nagashima, K
    Matsuda, M
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (33) : 25488 - 25493
  • [3] The Mystery of Rap1 Suppression of Oncogenic Ras
    Nussinov, Ruth
    Jang, Hyunbum
    Zhang, Mingzhen
    Tsai, Chung-Jung
    Sablina, Anna A.
    [J]. TRENDS IN CANCER, 2020, 6 (05): : 369 - 379
  • [4] Differential spatio-temporal activation of Ras and Rap1 in living cells
    Philips, M
    Bivona, T
    [J]. FASEB JOURNAL, 2004, 18 (08): : C1 - C1
  • [5] Ras and Rap1: A tale of two GTPases
    Shah, Seema
    Brock, Ethan J.
    Ji, Kyungmin
    Mattingly, Raymond R.
    [J]. SEMINARS IN CANCER BIOLOGY, 2019, 54 : 29 - 39
  • [6] Rap1 activation in response to cAMP occurs downstream of Ras activation during Dictyostelium aggregation
    Bolourani, Parvin
    Spiegelman, George B.
    Weeks, Gerald
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (16) : 10232 - 10240
  • [7] The small GTPases Ras and Rap1 bind to and control TORC2 activity
    Ankita Khanna
    Pouya Lotfi
    Anita J. Chavan
    Nieves M. Montaño
    Parvin Bolourani
    Gerald Weeks
    Zhouxin Shen
    Steven P. Briggs
    Henderikus Pots
    Peter J. M. Van Haastert
    Arjan Kortholt
    Pascale G. Charest
    [J]. Scientific Reports, 6
  • [8] The small GTPases Ras and Rap1 bind to and control TORC2 activity
    Khanna, Ankita
    Lotfi, Pouya
    Chavan, Anita J.
    Montano, Nieves M.
    Bolourani, Parvin
    Weeks, Gerald
    Shen, Zhouxin
    Briggs, Steven P.
    Pots, Henderikus
    Van Haastert, Peter J. M.
    Kortholt, Arjan
    Charest, Pascale G.
    [J]. SCIENTIFIC REPORTS, 2016, 6
  • [9] SHANK proteins limit integrin activation by directly interacting with Rap1 and R-Ras
    Johanna Lilja
    Thomas Zacharchenko
    Maria Georgiadou
    Guillaume Jacquemet
    Nicola De Franceschi
    Emilia Peuhu
    Hellyeh Hamidi
    Jeroen Pouwels
    Victoria Martens
    Fatemeh Hassani Nia
    Malte Beifuss
    Tobias Boeckers
    Hans-Juergen Kreienkamp
    Igor L. Barsukov
    Johanna Ivaska
    [J]. Nature Cell Biology, 2017, 19 : 292 - 305
  • [10] Spatio-temporal images of growth-factor-induced activation of Ras and Rap1
    Naoki Mochizuki
    Shigeko Yamashita
    Kazuo Kurokawa
    Yusuke Ohba
    Takeharu Nagai
    Atsushi Miyawaki
    Michiyuki Matsuda
    [J]. Nature, 2001, 411 : 1065 - 1068