Phenolic and biological activities of Vitex trifolia aerials parts

Vitex trifolia (Family: Verbenaceae) grows as an herbaceous plant in Egypt. Most of the Verbenaceae plants contain phenolic compounds which have important pharmacologically properties. V. trifolia aerial parts methanol extract was fractionated by repeated column chromatographic separation to obtain a phenyl ethanoid which isolated for the first time from the genus Vitex along with five phenolic metabolites. The identification and structure elucidation of the isolated compounds were based on chemical and spectral data (UV, ESI-MS, (HNMR)-H-1, (CNMR)-C-13, HMQC and HMBC) and also by direct comparison with respectively published data. Cytotoxic activities of the plant aerial parts extracts (methanol, ethyl acetate and chloroform) have been studied herein for the first time using, brine shrimp bioassay method (LC50 values 140 mg ml(-1), 165 mg ml(-1) and 180 mg ml(-1), respectively). Total phenolic content of different aerial parts extracts and the antioxidant activity of the major isolates has been studied as well. The radical scavenging activities of compounds 1 - 3 were measured and compound 1 have been identified as the most promising compound. [Salah EL-Kousy, Mona Mohamed, Shimaa Mohamed. Phenolic and biological activities of Vitex trifolia aerials parts. Life Sci J. 2012; 9(2): 670-677]. (ISSN: 1097-8135). http://www.lifesciencesite.com. 100