PROTEOMIC EVALUATION OF MCF-7 HUMAN BREAST CANCER CELLS AFTER TREATMENT WITH RETINOIC ACID ISOMERS: PRELIMINARY INSIGHTS

The specific aim of this work was exploration of molecular mechanisms in relation to therapy of human breast cancer. One of the efficient strategies to battle cancer is biomarker identification as an important tool in early cancer diagnosis and advisable treatment. Retinoids have been regarded as important therapeutic agents for many types of cancers, including human breast. The effects of 9-cis retinoic acid and all-trans retinoic acid in human MCF-7 breast cancer line have been investigated. The total cell proteins were extracted utilizing a commercially RIPA buffer (Radio-Immunoprecipitation Assay) and separated on 1D sodium dodecyl sulfate polyacrylamide gel electrophoresis (1D SDS-PAGE). The proteins were subsequently in-gel digested by trypsin and identified by matrix assisted laser desorption ionization technique with time of flight mass analyzer (MALDI-TOF/TOF).