An Amperometric Immunosensor Based on a Gold Nanoparticle-Diazonium Salt Modified Sensing Interface for the Detection of HbA1c in Human Blood

被引：37

作者：

Liu, Guozhen ^{[1
,2
]}

Iyengar, Sridhar G. ^{[3
]}

Gooding, J. Justin ^{[1
,2
]}

机构：

[1] Univ New S Wales, Sch Chem, Sydney, NSW 2052, Australia

[2] Univ New S Wales, Australian Ctr NanoMed, Sydney, NSW 2052, Australia

[3] AgaMatrix Inc, Salem, NH 03079 USA

来源：

ELECTROANALYSIS | 2013年 / 25卷 / 04期

基金：

澳大利亚研究理事会;

关键词：

Amperometric immunosensor; Aryl diazonium salts; Functionalized gold nanoparticles; HbA1c; ELECTRON-TRANSFER; ELECTROCHEMICAL IMMUNOBIOSENSOR; CARBON ELECTRODES;

D O I：

10.1002/elan.201200333

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An amperometric immunosensor for glycosylated hemoglobin (HbA1c) is reported. A glassy carbon electrode is modified with gold nanoparticles (AuNPs) bearing a ferrocene derivative and a glycosylated pentapeptide (GPP) as an epitope to which anti-HbA1c IgG can selectively bind. The rest of the electrode is passivated with an oligo(ethylene oxide) species to give the electrode resistant to nonspecific adsorption of proteins. Complexation of anti-HbA1c IgG with the surface bound epitope resulted in attenuation of the ferrocene electrochemistry. The immunosensor can detect HbA1c in the range of 4.6%15.1% of total hemoglobin in human blood by a competitive inhibition assay.

引用

页码：881 / 887

页数：7

共 12 条

[1] An Electrochemical Impedance Immunosensor Based on Gold Nanoparticle-Modified Electrodes for the Detection of HbA1c in Human Blood
Liu, Guozhen
Iyengar, Sridhar G.
Gooding, J. Justin
ELECTROANALYSIS, 2012, 24 (07) : 1509 - 1516
[2] An Electrochemical HbA1c Immunosensor With a Simple Electrode of Gold Nanoparticle Monolayer
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IEEE SENSORS JOURNAL, 2023, 23 (15) : 16579 - 16584
[3] A field effect transistor (FET)-based immunosensor for detection of HbA1c and Hb
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[4] A field effect transistor (FET)-based immunosensor for detection of HbA1c and Hb
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[5] Electrochemical aptasensor for ultrasensitive detection of glycated hemoglobin (HbA1c) using gold-modified SPCE
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[6] Developing a Sensing Platform based on Molecular Imprinting of HbA1c on Fe3O4 Nanoparticle Modified Screen-Printed Electrode
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[7] Zeta potential based colorimetric immunoassay for the direct detection of diabetic marker HbA1c using gold nanoprobes
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[8] A glycosylated hemoglobin (HbA1c) biosensing in human whole blood based on the boronate-diol interaction
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[9] Development of aptamer based colorimetric assay for whole blood glycated hemoglobin (HbA1c) estimation using gold nanoparticles
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Biosensors and Bioelectronics: X, 2023, 13
[10] Bioelectrocatalytic detection of glycated hemoglobin (HbA1c) based on the competitive binding of target and signaling glycoproteins to a boronate-modified surface
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