Calcium Phosphate Transfection of Primary Hippocampal Neurons

被引：21
|
作者：
Sun, Miao [1 ]
Bernard, Laura P. [1 ]
DiBona, Victoria L. [1 ]
Wu, Qian [1 ]
Zhang, Huaye [1 ]
机构：
[1] Rutgers State Univ, Robert Wood Johnson Med Sch, Dept Neurosci & Cell Biol, Piscataway, NJ 08855 USA
来源：
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2013年 / 81期
关键词：
Neuroscience; Issue; 81; Primary hippocampal neuron; calcium phosphate transfection; Coculture; astroglial cells; DNA; EFFICIENCY;
D O I：
10.3791/50808
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Calcium phosphate precipitation is a convenient and economical method for transfection of cultured cells. With optimization, it is possible to use this method on hard-to-transfect cells like primary neurons. Here we describe our detailed protocol for calcium phosphate transfection of hippocampal neurons cocultured with astroglial cells.
引用
收藏
页数：7
相关论文
共 50 条
  • [1] Chemically controlled formation of a DNA/calcium phosphate coprecipitate: Application for transfection of mature hippocampal neurons
    Goetze, B
    Grunewald, B
    Baldassa, S
    Kiebler, M
    JOURNAL OF NEUROBIOLOGY, 2004, 60 (04): : 517 - 525
  • [2] VACCINIA VIRUS TRANSFECTION OF HIPPOCAMPAL SLICE NEURONS
    PETTIT, OL
    KOOTHAN, T
    LIAO, DZ
    MALINOW, R
    NEURON, 1995, 14 (04) : 685 - 688
  • [3] Calcium phosphate nanoparticles for the transfection of cells
    Kovtun, Anna
    Heumann, R.
    Epple, Matthias
    BIO-MEDICAL MATERIALS AND ENGINEERING, 2009, 19 (2-3) : 241 - 247
  • [4] Calcium phosphate-mediated transfection of primary cultured brain neurons using GFP expression as a marker: application for single neuron electrophysiology
    Watanabe, SY
    Albsoul-Younes, AM
    Kawano, T
    Itoh, H
    Kaziro, Y
    Nakajima, S
    Nakajima, Y
    NEUROSCIENCE RESEARCH, 1999, 33 (01) : 71 - 78
  • [5] Double transfection into primary dissociated neurons
    Eguchi, Megumi
    Yamaguchi, Shun
    JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2007, 103 (05) : 497 - 499
  • [6] Transfection of adherent and suspended cells by calcium phosphate
    Jordan, M
    Wurm, F
    METHODS, 2004, 33 (02) : 136 - 143
  • [7] Calcium phosphate - mediated transfection of eukaryotic cells
    不详
    NATURE METHODS, 2005, 2 (04) : 319 - 320
  • [8] Calcium phosphate–mediated transfection of eukaryotic cells
    Nature Methods, 2005, 2 : 319 - 320
  • [9] High-Efficiency Transfection of Short Hairpin RNAs-Encoding Plasmids Into Primary Hippocampal Neurons
    Zeitelhofer, Manuel
    Karra, Daniela
    Vessey, John P.
    Jaskic, Elmir
    Macchi, Paolo
    Thomas, Sabine
    Riefler, Julia
    Kiebler, Michael
    Dahm, Ralf
    JOURNAL OF NEUROSCIENCE RESEARCH, 2009, 87 (01) : 289 - 300
  • [10] Thrombin receptor on rat primary hippocampal neurons: Coupled calcium and cAMP responses
    Yang, YX
    Akiyama, H
    Fenton, JW
    Brewer, GJ
    BRAIN RESEARCH, 1997, 761 (01) : 11 - 18
12345