Turbidimetric method for quantitative determination of Triton X-100 with silicotungstic acid

被引：5

作者：

Lagutina, LS ^{[1
]}

Sholts, KF ^{[1
]}

机构：

[1] Russian Acad Sci, Bach Inst Biochem, Moscow 117071, Russia

来源：

APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2002年 / 38卷 / 03期

关键词：

Calibration Curve; Complex Formation; Quantitative Determination; Complex Nature; Longe Wavelength;

D O I：

10.1023/A:1015439912362

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The formation of Triton X-100-silicotungstic acid complex was studied. Quantitative turbidimetric determination of the detergent based on this process was suggested. This method allows us to determine the complex formation at any wavelength in the range from 350 (epsilon(350) = 15 600 cm(-1) M-1) to 600 nm (epsilon(600) = 9090 cm(-1) M-1). The calibration curve for Triton X-100 recorded at 350 nm is linear in the concentration range of 0 to 30 mug/ml. A sigmoid calibration curve was observed at longer wavelengths. A linear fragment of the calibration curve recorded at 600 nm was found at a concentration of Triton X-100 of about 5 mug/ml. The complex nature of calibration curves can be explained by the heterogeneity of the complex dispersion.

引用

页码：294 / 297

页数：4

共 50 条

[1] Turbidimetric Method for Quantitative Determination of Triton X-100 with Silicotungstic Acid
L. S. Lagutina
K. F. Sholts
[J]. Applied Biochemistry and Microbiology, 2002, 38 : 294 - 297
[2] Determination of the micellar properties of Triton X-100 by voltammetry method
Liu, TP
Guo, R
Song, GP
[J]. JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY, 1999, 20 (04) : 1205 - 1221
[3] A SIMPLE TURBIDIMETRIC METHOD FOR THE DETERMINATION OF MICROGRAM QUANTITIES OF TRITION X-100
YOSHIDA, K
ISHIHARA, K
OHARA, S
AZUUMI, Y
HOTTA, K
[J]. ANALYTICAL BIOCHEMISTRY, 1980, 108 (01) : 162 - 165
[4] LOWRY DETERMINATION OF PROTEIN IN PRESENCE OF TRITON X-100
WANG, CS
SMITH, RL
[J]. ANALYTICAL BIOCHEMISTRY, 1975, 63 (02) : 414 - 417
[5] Dimensions and Orientation of Triton X-100 Micelles in Aqueous Solutions, According to Turbidimetric Data
O. A. Fedyaeva
E. G. Poshelyuzhnaya
[J]. Russian Journal of Physical Chemistry A, 2019, 93 : 2559 - 2561
[6] Dimensions and Orientation of Triton X-100 Micelles in Aqueous Solutions, According to Turbidimetric Data
Fedyaeva, O. A.
Poshelyuzhnaya, E. G.
[J]. RUSSIAN JOURNAL OF PHYSICAL CHEMISTRY A, 2019, 93 (12) : 2559 - 2561
[7] DETERMINATION OF ACID-LABILE SULFIDE IN SUBCHLOROPLAST PARTICLES CONTAINING TRITON X-100
GOLBECK, JH
SANPIETRO, A
[J]. ANALYTICAL BIOCHEMISTRY, 1976, 73 (02) : 539 - 542
[8] Adsorptive voltammetric determination of chlorpromazine in the presence of triton X-100
Zhang, ZQ
Chen, ZG
Yang, ZG
Zhang, H
[J]. MICROCHEMICAL JOURNAL, 1996, 53 (03) : 282 - 289
[9] DETERMINATION OF INORGANIC-PHOSPHATE IN PRESENCE OF TRITON X-100
SEE, YP
FITT, PS
[J]. ANALYTICAL BIOCHEMISTRY, 1972, 49 (02) : 430 - &
[10] ELIMINATION OF THE TRITON X-100 EFFECT IN THE DETERMINATION OF INORGANIC PHOSPHORUS
MALYGIN, AE
GAVRILOVA, NI
[J]. LABORATORNOE DELO, 1984, (02): : 122 - 124

← 1 2 3 4 5 →