Fractionation of apple procyanidins by size-exclusion chromatography

被引：93
|
作者：
Yanagida, A
Kanda, T
Shoji, T
Ohnishi-Kameyama, M
Nagata, T
机构：
[1] Minist Agr Forestry & Fisheries, Natl Food Res Inst, Tsukuba, Ibaraki 3058642, Japan
[2] Nikka Whisky Distilling Co Ltd, Inst Prod Res & Dev, Chiba 2770033, Japan
来源：
JOURNAL OF CHROMATOGRAPHY A | 1999年 / 855卷 / 01期
关键词：
apples; fruits; fruit juices; food analysis; procyanidins; tannins;
D O I：
10.1016/S0021-9673(99)00684-6
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Oligomeric constituents of apple procyanidins were fractionated by size-exclusion chromatography using a TSKgel Toyopearl HW-40F column. The best separation was obtained using a mobile phase of acetone-8 M urea (6:4; adjusted to pH 2) at a flow-rate of 1.0 ml/min. In this chromatographic system, the use of 8 M urea in the mobile phase resulted in a molecular sieve effect without any surface affinity interaction between the gel beads and the procyanidin molecules. Each fraction obtained was examined by reversed-phase high-performance liquid chromatography and time-of-flight mass spectrometry. The order of elution of the procyanidins from the column was coincident with their degree of polymerization. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：181 / 190
页数：10
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