Fast genetic identification of the Beluga sturgeon and its sought-after caviar to stem illegal trade

被引：21

作者：

Boscari, Elisa ^{[1
]}

Vitulo, Nicola ^{[2
]}

Ludwig, Arne ^{[3
]}

Caruso, Chiara ^{[1
]}

Mugue, Nikolai S. ^{[4
,5
]}

Suciu, Radu ^{[6
]}

Onara, Dalia F. ^{[6
]}

Papetti, Chiara ^{[1
]}

Marino, Ilaria A. M. ^{[1
]}

Zane, Lorenzo ^{[1
]}

Congiu, Leonardo ^{[1
]}

机构：

[1] Univ Padua, Dept Biol, I-35131 Padua, Italy

[2] Univ Verona, Dept Biotechnol, I-37134 Verona, Italy

[3] Leibniz Inst Zoo & Wildlife Res, D-10252 Berlin, Germany

[4] Russian Fed Res Inst Fisheries & Oceanog, Moscow 107140, Russia

[5] RAS, Inst Dev Biol, Moscow 117808, Russia

[6] Danube Delta Natl Inst Res & Dev, Tulcea 820112, Romania

来源：

FOOD CONTROL | 2017年 / 75卷

关键词：

Sturgeon; Huso huso; RP2S6; Bester; Species identification; Hybrids; ACIPENSER-NACCARII BONAPARTE; HUSO-HUSO LINNAEUS; ADRIATIC STURGEON; GENOME; TRANSCRIPTOME; RUTHENUS; AFLP;

D O I：

10.1016/j.foodcont.2016.11.039

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Sturgeons are well known for the delicacy of their eggs, the caviar, one of the most valuable products on the food market. The high price of caviar led in the past to a severe overharvest of wild sturgeon species and to an increase in trade of counterfeit products sold with impunity in spite of the strict trade limitations. A priority in the effort to reduce illegal trading is the development of genetic tools in order to identify the species of traded products using a standardized, cheap and rapid approach. We developed the first genetic nuclear marker for the identification of the Beluga sturgeon (Huso huso), the most sought-after caviar producer. We explored the interspecific variability at the setond intron of the nuclear S6 Ribosomal Protein (RP2S6), selected among 1867 introns, predicted by aligning the transcriptome of 3 sturgeon species with 3 complete fish genomes. The Beluga-specific SNP was identified by cloning and sequencing RP2S6 in 65 individuals of 11 species, validated on 341 additional individuals and tested on 18 caviar samples. Diagnostic primers designed on the SNP successfully amplified the expected band in all Beluga specimens while no PCR product was obtained from other pure species. The marker can also contribute to the identification of interspecific hybrids in which the Beluga is one of the parent species, such as in the case of the Bester, which produces one of the most mislabeled caviars in trade. The complete identification power on this highly relevant species and the proved efficacy on caviar samples represent an essential progress towards a standardized panel of nuclear markers for the control of illegal poaching, smuggling and mislabeling of sturgeons and their products. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：145 / 152

页数：8