Use of high-resolution techniques for the characterization of clotting factor VIII

被引:7
|
作者
Pock, K
Rizzi, A
Josic, D
机构
[1] Octapharma Pharmazeut Prod GesmbH, A-1100 Vienna, Austria
[2] Univ Vienna, Inst Analyt Chem, A-1090 Vienna, Austria
关键词
reviews; factor VIII; proteins; glycoproteins; von Willebrand factor;
D O I
10.1016/S0021-9673(99)00620-2
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Dealing with the structural characterization of clotting factor VIII (FVIII) requires the application of several high-resolution analytical techniques. Besides the analytical point of view, a detailed knowledge of FVIII structure, production and therapeutic application is necessary. This review gives an overview of most of the currently applied analytical methods and how they deal with the complex analytical problem, investigating FVIII in a sample matrix containing large amounts of accompanying plasma proteins. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:175 / 188
页数:14
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