Functional characterization of the β-amyrin synthase gene involved in platycoside biosynthesis in Platycodon grandiflorum

被引:5
|
作者
Um, Yurry [1 ]
Jin, Mei Lan [1 ]
Lee, Dae Young [1 ]
Kim, Chang Kug [2 ]
Hong, Chang Pyo [3 ]
Lee, Yi [4 ]
Kim, Ok Tae [1 ]
机构
[1] RDA, Dept Herbal Crop Res, Natl Inst Hort & Herbal Sci, Eumseong 27709, South Korea
[2] RDA, NAAS, Genom Div, Jeonju 54874, South Korea
[3] Theragen Etex, Theragen Etex Bio Inst, Suwon 16229, South Korea
[4] Chungbuk Natl Univ, Dept Ind Plant Sci & Technol, Cheongju 28644, South Korea
关键词
beta-amyrin synthase; oxidosqualene cyclase; platycosides; triterpenoids; OXIDOSQUALENE CYCLASES; TRITERPENE BIOSYNTHESIS; STERIC BULK; CLONING;
D O I
10.1007/s13580-017-0054-z
中图分类号
S6 [园艺];
学科分类号
0902 ;
摘要
Platycodon grandiflorum A. DC. contains a variety of triterpene saponins induced from the base structure of oleanane-type in roots. The beta-amyrin synthase (bAS) plays an important role in controlling the biosynthesis of triterpene saponins and we obtained the full-length cDNA of the gene, which is named PlgOSC1 in P. grandiflorum. To identify the PlgOSC1 products, we expressed it in heterologous yeast cells. Results of GC-MS analysis showed a unique peak that was consistent with that of the authentic beta-amyrin standard with exactly the same retention time. Furthermore, the pattern of MS fragments of the peak was also exactly the same as that of the beta-amyrin. In qRT-PCR analysis of four organs of P. grandiflorum, no difference in PlgOSC1 expression level was observed; therefore, platycosides derived from beta-amyrin might be present in all P. grandiflorum tissues. In conclusion, PlgOSC1 encodes a bAS enzyme that catalyzes the first committed step of platycosides biosynthesis in P. grandiflorum.
引用
收藏
页码:613 / 619
页数:7
相关论文
共 50 条
  • [1] Functional characterization of the β-amyrin synthase gene involved in platycoside biosynthesis in Platycodon grandiflorum
    Yurry Um
    Mei Lan Jin
    Dae Young Lee
    Chang Kug Kim
    Chang Pyo Hong
    Yi Lee
    Ok Tae Kim
    [J]. Horticulture, Environment, and Biotechnology, 2017, 58 : 613 - 619
  • [2] Molecular cloning, functional characterization and expression of the β-amyrin synthase gene involved in saikosaponin biosynthesis in Bupleurum chinense DC.
    Mao, Yanping
    Chen, Hua
    Zhao, Jun
    Li, Yuchan
    Feng, Liang
    Yang, Yuping
    Zhang, Yiguan
    Wei, Ping
    Hou, Dabin
    [J]. JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY, 2023, 32 (02) : 284 - 295
  • [3] Molecular cloning, functional characterization and expression of the β-amyrin synthase gene involved in saikosaponin biosynthesis in Bupleurum chinense DC.
    Yanping Mao
    Hua Chen
    Jun Zhao
    Yuchan Li
    Liang Feng
    Yuping Yang
    Yiguan Zhang
    Ping Wei
    Dabin Hou
    [J]. Journal of Plant Biochemistry and Biotechnology, 2023, 32 : 284 - 295
  • [4] Functional characterization of amyrin synthase involved in ursolic acid biosynthesis in Catharanthus roseus leaf epidermis
    Yu, Fang
    Thamm, Antje M. K.
    Reed, Darwin
    Villa-Ruano, Nemesio
    Quesada, Alfonso Lara
    Lozoya Gloria, Edmundo
    Covello, Patrick
    De Luca, Vincenzo
    [J]. PHYTOCHEMISTRY, 2013, 91 : 122 - 127
  • [5] Functional analysis of β-amyrin synthase gene in ginsenoside biosynthesis by RNA interference
    Che Zhao
    Tianhui Xu
    Yanlong Liang
    Shoujing Zhao
    Luquan Ren
    Qian Wang
    Bo Dou
    [J]. Plant Cell Reports, 2015, 34 : 1307 - 1315
  • [6] Functional analysis of β-amyrin synthase gene in ginsenoside biosynthesis by RNA interference
    Zhao, Che
    Xu, Tianhui
    Liang, Yanlong
    Zhao, Shoujing
    Ren, Luquan
    Wang, Qian
    Dou, Bo
    [J]. PLANT CELL REPORTS, 2015, 34 (08) : 1307 - 1315
  • [7] Isolation and characterization of an oxidosqualene cyclase gene encoding a β-amyrin synthase involved in Polygala tenuifolia Willd. saponin biosynthesis
    Mei Lan Jin
    Dae Young Lee
    Yurry Um
    Jeong Hoon Lee
    Chun Geun Park
    Reinhard Jetter
    Ok Tae Kim
    [J]. Plant Cell Reports, 2014, 33 : 511 - 519
  • [8] Isolation and characterization of an oxidosqualene cyclase gene encoding a β-amyrin synthase involved in Polygala tenuifolia Willd. saponin biosynthesis
    Jin, Mei Lan
    Lee, Dae Young
    Um, Yurry
    Lee, Jeong Hoon
    Park, Chun Geun
    Jetter, Reinhard
    Kim, Ok Tae
    [J]. PLANT CELL REPORTS, 2014, 33 (03) : 511 - 519
  • [9] Cloning and functional characterization of the β-amyrin synthase gene from Bupleurum chinense
    Li, J. -C.
    Wang, C.
    Qi, W. -T.
    Liu, C. -L.
    [J]. BIOLOGIA PLANTARUM, 2020, 64 : 314 - 319
  • [10] Candidate Genes Involved in the Biosynthesis of Triterpenoid Saponins in Platycodon grandiflorum Identified by Transcriptome Analysis
    Ma, Chun-Hua
    Gao, Zheng-Jie
    Zhang, Jia-Jin
    Zhang, Wei
    Shao, Jian-Hui
    Hai, Mei-Rong
    Chen, Jun-Wen
    Yang, Sheng-Chao
    Zhang, Guang-Hui
    [J]. FRONTIERS IN PLANT SCIENCE, 2016, 7