Compact in-line lensfree digital holographic microscope

被引:18
|
作者
Rostykus, Manon [1 ]
Soulez, Ferreol [2 ,3 ]
Unser, Michael [2 ]
Moser, Christophe [1 ]
机构
[1] Ecole Polytech Fed Lausanne, Lab Appl Photon Devices, Lausanne, Switzerland
[2] Ecole Polytech Fed Lausanne, Biomed Imaging Grp, Lausanne, Switzerland
[3] Univ Lyon 1, Univ Lyon, Ens Lyon, CNRS,Ctr Rech Astrophys Lyon UMR5574, F-69230 St Genis Laval, France
基金
欧洲研究理事会;
关键词
TWIN-IMAGE ELIMINATION; PHASE RETRIEVAL; CELL MORPHOMETRY; REFRACTIVE-INDEX; ALGORITHMS; TOMOGRAPHY;
D O I
10.1016/j.ymeth.2017.11.008
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Phase imaging provides intensity contrast to visualize transparent samples such as found in biology without any staining. Among them, digital holographic microscopy (DHM) is a well-known quantitative phase method. Lensfree implementations of DHMs offer the added advantage to provide large field of views (several mm(2) compared to several hundred mu m(2)) and more compact setups that traditional DHM which have high quality microscope objectives. In this article, a lensfree DHM is presented using a side illumination technique in order to further reduce the device size. Its practical use is described and results on a transparent (phase only) sample are shown. (C) 2017 The Authors. Published by Elsevier Inc.
引用
收藏
页码:17 / 23
页数:7
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