High-resolution melting analysis for rapid detection of linezolid resistance (mediated by G2576T mutation) in Staphylococcus epidermidis

被引：10
|
作者：
Gabriel, Emma M. [1 ]
Douarre, Pierre E. [1 ]
Fitzgibbon, Siobhan [2 ]
Clair, James [2 ]
Lucey, Brigid [1 ,3 ]
Coffey, Aidan [1 ]
O'Mahony, Jim M. [1 ]
机构：
[1] Cork Inst Technol, Cork, Ireland
[2] Mercy Univ Hosp, Cork, Ireland
[3] Cork Univ Hosp, Cork, Ireland
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2012年 / 90卷 / 02期
关键词：
High resolution melting; Staphylococcus epidermidis; Linezolid resistance; G2576T mutation; MECHANISMS;
D O I：
10.1016/j.mimet.2012.04.002
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We describe a novel HRM-PCR (high-resolution melting) assay capable of the accurate identification of the G2576T point mutation in domain V of the 235 rRNA genes attributed to linezolid resistance in Staphylococcus epidermidis. This rapid method demonstrated 100% correlation with the previously established NheI restriction digest assay. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：134 / 136
页数：3
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