Characterization of the expression of Na+, K+-ATPase subunits in human breast cancer cells

被引：0
|
作者：
Dias M., Almeida [1 ]
Capella M. A., Marques [1 ]
Lopes, A. G. [1 ]
机构：
[1] Inst Biofis Carlos Chagas Filho Brazil, Rio De Janeiro, Brazil
来源：
ICCB 2012: PROCEEDINGS OF THE 10TH INTERNATIONAL CONGRESS ON CELL BIOLOGY | 2013年
关键词：
Na+; K+-ATPase; breast cancer; MCF7; MDA-MB-231; TIGHT JUNCTIONS; E-CADHERIN; NA; K-ATPASE; ADHERENS; OUABAIN;
D O I：
暂无
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Background: It is well established that the known Na+, K+-ATPase has cell adhesion molecule role in adherens junctions [16]. In so, beta subunit acts on maturation and membrane targeting of a catalytic subunit. Studies have demonstrated the importance of the sodium permeability in tumorigenesis, but there's no study of Na+, K+-ATPase alpha and beta subunit's expression. The aim of the present study is to characterize the expression of Na+, K+-ATPase subunits in human breast cancer cells. Methods and Results: To observe the expression of alpha 1 and beta 1 in MCF-7, MDA-231 and MDCK cells were seeded in 6 well plates for 24, 48 and 72 hours. The measurement of of alpha 1 and beta 1 expression by Western Blotting showed that tumor cell lines do not express beta 1 subunit. Conclusion: Our results indicate that there's a distinct adhesion mechanism in tumor cells, which should explain metastasis.
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页码：7 / 10
页数：4
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