Production with a High Yield of Bacterial Cellulose Nanocrystals by Enzymatic Hydrolysis

Bacterial cellulose nanocrystals are highly crystalline structures with nanoscopic scale dimensions that have received increased attention in the nanocomposites area. Its properties, such as large surface area, low density, mechanical strength and ease of modification, are attractive to the preparation many kinds of nanomaterials applied multifunctional in various fields. Besides, the cellulose nanocrystals are from abundant and renewable sources that are biodegradable. An altemative method is to obtain bacterial cellulose nanocrystal by enzymatic hydrolysis because it is, less expensive, it does not use chemicals and it requires much less energy. In this sense, the primary objective of this study was to produce bacterial cellulose using glycerol as a carbon source and isolate nanocrystals from bacterial cellulose using the enzymatic hydrolysis. This study also investigated the yield of nanocrystals depending on the weight of the bacterial cellulose hydrogel, keeping constant some enzymes. The study shows us that the enzymatic method has the best performance when using cellulose hydrogel 2 g to 40 mu L cellulase enzyme (endoglucanase) and 1 mL of citrate buffer. Also, it was observed that the yield of nanocrystals decrease with increasing time required for the hydrolysis.