Grape Marc Extract-Induced Defense Reactions and Protection against Phytophthora parasitica Are Impaired in NahG Tobacco Plants

被引：8

作者：

Benouaret, Razik ^{[1
,2
]}

Goupil, Pascale ^{[1
,2
]}

机构：

[1] Blaise Pascal Univ, Clermont Univ, UMR PIAF 547, BP 10448, Clermont Ferrand, France

[2] INRA, UMR PIAF 547, Clermont Ferrand, France

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2015年 / 63卷 / 30期

关键词：

grape marc extract; NahG; Phytophthora parasitica; protection; salicylic acid; WINERY BY-PRODUCTS; SALICYLIC-ACID; INDUCED RESISTANCE; CELL-DEATH; POLYPHENOLS; INDUCTION;

D O I：

10.1021/acs.jafc.5b01740

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Grape marc extract (GME) acts as an elicitor of plant defense responses. This study analyzed GME-induced plant defense reactions in NahG transgenic tobacco. Leaf infiltration of NahG leaves revealed HR-like reactions with reduced lesions and weak deployment of autofluorescent compounds in the surrounding infiltrated tissues. The beta-1,3-glucanase PR2-, endochitinase PR3-, and osmotin PR5-target transcript levels were strongly lowered in NahG leaves, and the mutant failed to accumulate the antimicrobial PR1 transcripts. GME-induced protection against Phytophthora parasitica var. nicotianae (Ppn) was evaluated on tobacco leaves. The antimicrobial properties of GME against Ppn were evidenced using a range of in vitro tests. GME-sprayed wild-type leaves showed reduced infection areas, whereas GME failed to induce a protective effect against Ppn in NahG leaves. The results suggest that GME-induced plant defense reactions in tobacco plants was mediated by salicylic acid (SA) and that GME-induced protection against Ppn could be the combined result of antimicrobial and defense actions.

引用

页码：6653 / 6659

页数：7