Ecological risk assessment of neem-based pesticides

A tiered process was used to evaluate the risks Of pure azadirachtin (AZA) and two neem-based insecticides (Neemix(TM) and Bioneem(TM) I on six aquatic animals [crayfish (Procambarus clarkii), white shrimp (Penaeus setiferus), grass shrimp (Palaemonetes pugio), blue crabs (Callinectes sapidus), water fleas (Daphnia pulex), and mosquito larvae (Culex quinquefasciatus)] through short term acute toxicity tests. The risk Was calculated Using the level of concern endpoints (Q values) and relative hazard index (RHI) for acute and Chronic exposure scenarios. The Q values of' Neemix(TM), Bioneem(TM), and pure AZA derived from acute exposure tests indicated that D. pulex is the only sensitive species to the test pesticides. Furthermore, the RHI values of Neemix(TM) and Bioneem(TM) for D. pule-v were above the critical limit of 10 indicating that these pesticides may pose a moderate hazard to this species and related crustaceans in acute exposure scenarios. The RHI values the two pesticides and pure AZA were all below the critical limit of 10 for P. clarkii, P. setiferus, P. pugio, C. sapidus, and C. quinquefasciatus. The aquatic risk assessment process showed that the risk values of tested pesticides did not exceed the criteria, and therefore, no ecological hazard is likely to result from their use.