Six muscles were seamed out randomly from Hanwoo carcasses (n = 12) of each quality grade (quality grades 1, 2 and 3). Samples were analysed for their total and soluble collagen contents, IMCT (intramuscular connective tissue) and Warner-Bratzler shear force (WBSF). Simple correlation (n = 21) was determined for WBSF among major muscles. For LT (longissimus thoracis), total collagen content was significantly higher (p < 0.05) for quality grade 3 than those for quality grades 1 and 2. For semitendinosus (ST), semimembranosus (SM), psoas major (PM) and serratus ventralis (SV), total collagen content of quality grade 1 was lowest (p < 0.05) of all quality grades. IMCT shear force for gluteus medius (GM) decreased (p < 0.05) with better quality grade, and those for other muscles, with the exception of GM, were higher (p < 0.05) for quality grade 3 than for quality grades I and 2. WBSF values showed GM and LT to be decreased (p < 0.05) with better quality grade, and PM to be higher (p < 0.05) for quality grade 3 than those for quality grades 1 and 2. SM, ST and SV from quality grade I had lower (p < 0.05) WBSF value than those from quality grades 2 and 3. Total collagen content of ST was highest (p < 0.05) of all muscles, whereas that of PM was lowest (p < 0.05). Soluble collagen contents of LT and SV from quality grades 1 and 2 were, in general, higher (p < 0.05) than other muscles, but that of SM was lowest (p < 0.05). ST and SM had higher (p < 0.05) WBSF values for three quality grades when compared to other muscles, whereas PM was lowest (p < 0.05). LT had the strongest simple correlation with SV (r = 0.78) and GM (r = 0.77), and SM had the strongest correlation with ST (r = 0.73) and LT (r = 0.73). Also, PM had the strongest correlation with SV (r = 0.62).