Chemical stabilization of recombinant horseradish peroxidase

被引：0
|
作者：
OBrien, AM
Fagain, CO
机构：
来源：
BIOTECHNOLOGY TECHNIQUES | 1996年 / 10卷 / 12期
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D O I：
暂无
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Unglycosylated recombinant horseradish peroxidase (HRP C*) had a half life of 21 minutes at 65 degrees C compared with only 5 minutes for the plant enzyme (HRP). The half life of HRP C* at 65 degrees C increased by 5-fold following modification with ethylene glycol bis(succinic acid N-hydroxysuccinimide ester). Tolerance to 60% 1,4-dioxan also increased whilst tolerance to 30% dimethylformamide was unchanged.
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页码：905 / 910
页数：6
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