Genetic diversity analysis of Cynodon dactylon (bermudagrass) accessions and cultivars from different countries based on ISSR and SSR markers

被引:33
|
作者
Wang, Zhiyong [1 ,2 ,3 ,4 ]
Liao, Li [4 ]
Yuan, Xuejun [5 ]
Guo, Hailin [1 ,2 ]
Guo, Aigui [1 ,2 ]
Liu, Jianxiu [1 ,2 ]
机构
[1] Inst Bot, Nanjing 210014, Jiangsu, Peoples R China
[2] Chinese Acad Sci, Nanjing 210014, Jiangsu, Peoples R China
[3] Hainan Univ, Key Lab Protect & Dev Utilizat Trop Crop Germplas, Minist Educ, Haikou 570228, Peoples R China
[4] Hainan Univ, Coll Agron, Haikou 570228, Peoples R China
[5] Qiongzhou Univ, Coll Life Sci, Sanya 572000, Peoples R China
基金
中国国家自然科学基金;
关键词
Cynodon dactylon var. dactylon; Genetic diversity; Germplasm; ISSR marker; SSR marker; VAR; DACTYLON; REGISTRATION; SPP;
D O I
10.1016/j.bse.2012.09.001
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
ISSR and SSR markers were used to evaluate genetic diversity among 33 Cynodon dactylon accessions and 22 cultivars from four different countries in order to provide information on how to improve the utilization of bermudagrass germplasms. Eighty eight bands were amplified by nine SSR primer combinations and 236 bands were observed from 23 ISSR primers. The results showed that 97.7% of the SSR primers and 86.9% of the ISSR primers were polymorphic. The genetic similarity coefficients (GSC), gene diversity (He) and Shannon index (I) were 0.58-0.97, 0.27 and 0.41, respectively, for ISSR and 0.52-0.97, 0.29, and 0.43 for SSR. The UPGMA analysis clustered the 55 accessions (cultivars) into three groups. The cluster results produced by the ISSR data were close to the SSR data results. Analysis based on the combined ISSR and SSR data was more closely related to the geographical distribution of the tested germplasm. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码:108 / 115
页数:8
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