A new seed-transmissible begomovirus in bitter gourd (Momordica charantia L.)

被引：15

作者：

Manivannan, Kothandan ^{[1
]}

Renukadevi, Perumal ^{[1
]}

Malathi, Varagur Ganesan ^{[1
]}

Karthikeyan, Gandhi ^{[1
]}

Balakrishnan, Natarajan ^{[2
]}

机构：

[1] Tamil Nadu Agr Univ, Ctr Plant Protect Studies, Dept Plant Pathol, Coimbatore 641003, Tamil Nadu, India

[2] Tamil Nadu Agr Univ, Ctr Plant Mol Biol & Biotechnol, Coimbatore 641003, Tamil Nadu, India

来源：

MICROBIAL PATHOGENESIS | 2019年 / 128卷

关键词：

Begomovirus; Rolling circle amplification; Recombination; Seed transmission; LEAF-CURL-VIRUS; POLYMERASE-CHAIN-REACTION; MOLECULAR CHARACTERIZATION; MOSAIC-VIRUS; TAMIL-NADU; 1ST REPORT; TOMATO; DISEASE;

D O I：

10.1016/j.micpath.2018.12.036

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A begomovirus isolate collected from bitter gourd plants showing yellowing, puckering and stunting symptoms from Coimbatore district, Tamil Nadu, India was characterized. The full-length genome of the virus isolate was amplified by rolling circle amplification using phi29 DNA polymerase. The virus isolate exhibited 98% identity in the nucleotide sequence of DNA-A component with the Coccinia mosaic Virudhunagar virus (GenBank accession no. KY860899). The DNA-B component was very distinct and shared only 60% identity with the begomovirus, Coccinia mosaic Tamil Nadu virus (GenBank accession no. KM244719). The virus renamed as new species Bitter gourd yellow mosaic virus (BgYMV) was detected in seeds from infected plants and in the grow-out test seedlings by ELISA and virus-specific PCR. The seed infectivity was 79.16% and transmission rate to seedling was 32.05%. The virus titre as indicated by A(405) absorption value was high (0.854-11280) in different seed parts. Results clearly indicated seed transmission of the begomovirus, BgYMV.

引用

页码：82 / 89

页数：8

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