Identification and characterization of a highly alkaline and thermotolerant novel xylanase from Streptomyces sp.

被引：8
|
作者：
Thomas, Leya [1 ]
Sindhu, Raveendran [1 ]
Pandey, Ashok [1 ]
机构：
[1] Div Biotechnol, Natl Inst Interdisciplinary Sci & Technol, CSIR, Trivandrum 695019, Kerala, India
来源：
BIOLOGIA | 2013年 / 68卷 / 06期
关键词：
xylanase; Streptomyces sp; alkaline tolerance; thermo-tolerance; paper and pulp industry; INDUSTRIAL APPLICATIONS; MICROBIAL XYLANASES; ALIGNMENT; STRAIN;
D O I：
10.2478/s11756-013-0248-5
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Xylanases constitute an important industrial enzyme, which hydrolyzes the polysaccharide xylan. In this work, a novel Streptomyces strain producing cellulase-free xylanase was isolated from the soil samples collected from the mangrove forest of Kadalundi, Kerala, India. The strain produced unique enzyme, which exhibited optimal activity at pH 9.0 and tolerance up to pH 12.0. Media engineering was carried out to improve the enzyme production, which showed best enzyme production at 30A degrees C, medium pH 9.0 and incubation time of 48 h. Enzyme was highly thermo-tolerant up to 70A degrees C and alkaline tolerant. Partial gene amplification as well as partial purification of enzyme was carried out to characterize the enzyme. The unique features of the enzyme make it an ideal candidate for industrial application for paper and pulp industry.
引用
收藏
页码：1022 / 1027
页数：6
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