Are variable-number tandem repeats appropriate for genotyping Mycobacterium leprae?

被引：26
|
作者：
Monot, Marc [2 ]
Honore, Nadine [2 ]
Baliere, Charlotte [2 ]
Ji, Baohong [3 ]
Sow, Samba [4 ]
Brennan, Patrick J. [5 ]
Cole, Stewart T. [1 ,2 ]
机构：
[1] Ecole Polytech Fed Lausanne, Global Hlth Inst, CH-1015 Lausanne, Switzerland
[2] Inst Pasteur, Unite Genet Mol Bacterienne, Paris, France
[3] Univ Paris 06, Paris, France
[4] Ctr Natl Appui Lutte Contre Maladie, Bamako, Mali
[5] Colorado State Univ, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2008年 / 46卷 / 07期
基金：
美国国家卫生研究院;
关键词：
D O I：
10.1128/JCM.00239-08
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Comparative genomics analysis of the Tamil Nadu strain of Mycobacterium leprae has uncovered several polymorphic sites with potential as epidemiological tools. In this study we compared the stability of two different markers of genomic biodiversity of M. leprae in several biopsy samples isolated from the same leprosy patient. The first type comprises five different variable-number tandem repeats (VNTR), while the second is composed of three single nucleotide polymorphisms (SNP). Contrasting results were obtained, since no variation was seen in the SNP profiles of M. leprae from 42 patients from 7 different locations in Mali whereas the VNTR profiles varied considerably. Furthermore, since variation in the VNTR pattern was seen not only between different isolates of M. leprae but also between biopsy samples from the same patient, these VNTR may be too dynamic for use as epidemiological markers for leprosy.
引用
收藏
页码：2291 / 2297
页数：7
相关论文
共 50 条
  • [1] Rapid Variable-Number Tandem-Repeat Genotyping for Mycobacterium leprae Clinical Specimens
    Kimura, Miyako
    Sakamuri, Rama Murthy
    Groathouse, Nathan A.
    Rivoire, Becky L.
    Gingrich, David
    Krueger-Koplin, Susan
    Cho, Sang-Nae
    Brennan, Patrick J.
    Vissa, Varalakshmi
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (06) : 1757 - 1766
  • [2] Genotypic variation and stability of four variable-number tandem repeats and their suitability for discriminating strains of Mycobacterium leprae
    Truman, R
    Fontes, AB
    de Miranda, AB
    Suffys, P
    Gillis, T
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (06) : 2558 - 2565
  • [3] Polymorphism of variable-number tandem repeats at multiple loci in Mycobacterium tuberculosis
    Smittipat, N
    Billamas, P
    Palittapongarnpim, M
    Thong-On, A
    Temu, MM
    Thanakijcharoen, P
    Karnkawinpong, O
    Palittapongarnpim, P
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (10) : 5034 - 5043
  • [4] Characterisation of Short tandem repeats for genotyping Mycobacterium leprae
    Gillis, Thomas
    Vissa, Varalakshmi
    Matsuoka, Masanori
    Young, Saroj
    Richardus, Jan Hendrik
    Truman, Richard
    Hall, Barry
    Brennan, Patrick
    [J]. LEPROSY REVIEW, 2009, 80 (03) : 250 - 260
  • [5] Combination of Single Nucleotide Polymorphism and Variable-Number Tandem Repeats for Genotyping a Homogenous Population of Mycobacterium tuberculosis Beijing Strains in China
    Luo, Tao
    Yang, Chongguang
    Gagneux, Sebastien
    Gicquel, Brigitte
    Mei, Jian
    Gao, Qian
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2012, 50 (03) : 633 - 639
  • [6] Effective variable-number tandem repeats loci for discrimination of Mycobacterium tuberculosis isolated from Korea
    Park, Young Kil
    Kang, Hee Yoon
    Lee, Ji Hye
    Kim, Sa Rang
    [J]. EUROPEAN RESPIRATORY JOURNAL, 2012, 40
  • [7] Discriminatory potential of a novel set of Variable Number of Tandem Repeats for genotyping Mycobacterium marinum
    Sun, Gang
    Chen, Chao
    Li, Jing
    Gui, Xiaohong
    Li, Xueyuan
    Chen, Lin
    Peng, Feng
    Mei, Jian
    Gao, Qian
    [J]. VETERINARY MICROBIOLOGY, 2011, 152 (1-2) : 200 - 204
  • [8] Targeted Genotyping of Variable Number Tandem Repeats with AdVNTR
    Bakhtiari, Mehrdad
    Shleizer-Burko, Sharona
    Gymrek, Melissa
    Bansal, Vikas
    Bafna, Vineet
    [J]. RESEARCH IN COMPUTATIONAL MOLECULAR BIOLOGY, RECOMB 2018, 2018, 10812 : 243 - 244
  • [9] Targeted genotyping of variable number tandem repeats with adVNTR
    Bakhtiari, Mehrdad
    Shleizer-Burko, Sharona
    Gymrek, Melissa
    Bansal, Vikas
    Bafna, Vineet
    [J]. GENOME RESEARCH, 2018, 28 (11) : 1709 - 1719
  • [10] Molecular Typing of Mycobacterium bovis Strains Isolated in Italy from 2000 to 2006 and Evaluation of Variable-Number Tandem Repeats for Geographically Optimized Genotyping
    Boniotti, M. Beatrice
    Goria, Maria
    Loda, Daniela
    Garrone, Annalisa
    Benedetto, Alessandro
    Mondo, Alessandra
    Tisato, Ernesto
    Zanoni, Mariagrazia
    Zoppi, Simona
    Dondo, Alessandro
    Tagliabue, Silvia
    Bonora, Stefano
    Zanardi, Giorgio
    Pacciarini, M. Lodovica
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (03) : 636 - 644
12345