Adenosine Stimulate Proliferation and Migration in Triple Negative Breast Cancer Cells

Emerging evidence suggests that the adenosine (Ado) receptors may play crucial roles in tumor progression. Here, we show that Ado increases proliferation and migration in a triple negative breast cancer model, the MDA-MB 231 cell line. The use of specific agonists and antagonists evidenced that these effects depend on the activation of the A(2B) receptor, which then triggers an intracellular response mediated by the adenylate cyclase/PKA/cAMP signaling pathway. Ado also increases the expression of Na(V)1.5 channels, a potential bio-marker in breast cancer. Together, these data suggest important roles of the A(2B) receptors and Na(V)1.5 channels in the Ado-induced increase in proliferation and migration of the MDA-MB 231 cells.