PRODUCTION OF RECOMBINANT SERPINS IN ESCHERICHIA COLI

被引：3
|
作者：
Pearce, Mary C. [1 ]
Cabrita, Lisa D. [2 ]
机构：
[1] Monash Univ, Dept Biochem, Clayton, Vic 3168, Australia
[2] UCL, Dept Biol Mol & Struct, London, England
来源：
METHODS IN ENZYMOLOGY, VOL 501: SERPIN STRUCTURE AND EVOLUTION | 2011年 / 501卷
关键词：
PLASMINOGEN-ACTIVATOR INHIBITOR-1; EPITHELIUM-DERIVED FACTOR; CELL CARCINOMA ANTIGEN; CONFORMATIONAL-CHANGE; BIOLOGICAL-ACTIVITY; CRYSTAL-STRUCTURE; INCLUSION-BODIES; PURIFICATION; EXPRESSION; ENVIRONMENT;
D O I：
10.1016/B978-0-12-385950-1.00002-X
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Serpins represent a diverse family of proteins that are found in a wide range of organisms and cellular locations. In order to study them, most need to be produced recombinantly, as isolation from their source is not always possible. Due to their relatively uncomplicated structure (single domain, few posttranslational modifications), the serpins are usually amenable to expression in Escherichia coli, which offers a fast and cost-effective solution for the generation of large amounts of protein. This chapter outlines the general procedures used in the expression and subsequent purification of serpins in E. coli, with a particular focus on the methods used for antitrypsin, the archetypal member of the family.
引用
收藏
页码：13 / 28
页数：16
相关论文
共 50 条
  • [1] Production of recombinant serpins in Escherichia coli
    Bird, PI
    Pak, SC
    Worrall, DM
    Bottomley, SP
    [J]. METHODS, 2004, 32 (02) : 169 - 176
  • [2] Production of recombinant proteins in Escherichia coli
    Schumann, W
    Ferreira, LCS
    [J]. GENETICS AND MOLECULAR BIOLOGY, 2004, 27 (03) : 442 - 453
  • [3] The production of polyhydroxyalkanoates in recombinant Escherichia coli
    Li, Rui
    Zhang, Hanxing
    Qi, Qingsheng
    [J]. BIORESOURCE TECHNOLOGY, 2007, 98 (12) : 2313 - 2320
  • [4] RECOMBINANT PROTEIN PRODUCTION IN Escherichia coli
    Lara, Alvaro R.
    [J]. REVISTA MEXICANA DE INGENIERIA QUIMICA, 2011, 10 (02): : 209 - 223
  • [5] INTRACELLULAR PRODUCTION OF RECOMBINANT SERPINS IN YEAST
    Kaiserman, Dion
    Hitchen, Corinne
    Levina, Vita
    Bottomley, Stephen P.
    Bird, Phillip I.
    [J]. METHODS IN ENZYMOLOGY, VOL 501: SERPIN STRUCTURE AND EVOLUTION, 2011, 501 : 1 - 12
  • [6] VANILLIN PRODUCTION BY RECOMBINANT STRAINS OF ESCHERICHIA COLI
    Converti, Attilio
    de Faveri, Danilo
    Perego, Patrizia
    Barghini, Paolo
    Ruzzi, Maurizio
    Sene, Luciane
    [J]. BRAZILIAN JOURNAL OF MICROBIOLOGY, 2003, 34 : 108 - 110
  • [7] Production of recombinant xenotransplantation antigen in Escherichia coli
    Bettler, E
    Imberty, A
    Priem, B
    Chazalet, V
    Heyraud, A
    Joziasse, DH
    Geremia, RA
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 302 (03) : 620 - 624
  • [8] Strategies for the Production of Recombinant Protein in Escherichia coli
    Gopal Jee Gopal
    Awanish Kumar
    [J]. The Protein Journal, 2013, 32 : 419 - 425
  • [9] Production of phenylpropanoid amides in recombinant Escherichia coli
    Kang, Kiyoon
    Back, Kyoungwhan
    [J]. METABOLIC ENGINEERING, 2009, 11 (01) : 64 - 68
  • [10] Production of antifungal recombinant peptides in Escherichia coli
    Gavit, P
    Better, M
    [J]. JOURNAL OF BIOTECHNOLOGY, 2000, 79 (02) : 127 - 136
12345