Enzymatic synthesis of ascorbic acid fructoside by transfructosylation using levan fructotransferase

To enhance the stability of ascorbic acid, the glycosylation of ascorbic acid was studied using the transfructosylation activity of levan fructotransferase. When levan was used as glycosyl donor, a novel fructoside (ascorbic acid 2-fructoside) was formed by the transfructosylation activity of the levan fructotransferase. The production of ascorbic acid 2-fructoside was highly affected by the concentration of the fructosyl acceptor (ascorbic acid). When 35% of ascorbic acid and 2% of levan were incubated with LFTase of 0.5 unit/glevan at 37 degrees C for 85 h, a maximum 52 g/l of AA-2F was produced.