Stable Lipid Bilayers Based on Micro- and Nano-Fabrication as a Platform for Recording Ion-Channel Activities

被引：17

作者：

Hirano-Iwata, Ayumi ^{[1
,2
]}

Oshima, Azusa ^{[1
]}

Mozumi, Hideki ^{[1
]}

Kimura, Yasuo ^{[3
]}

Niwano, Michio ^{[1
,3
]}

机构：

[1] Tohoku Univ, Grad Sch Biomed Engn, Aoba Ku, 6-6 Aoba, Sendai, Miyagi 9808579, Japan

[2] Japan Sci & Technol Agcy JST, PRESTO, Kawaguchi, Saitama 3320012, Japan

[3] Tohoku Univ, Elect Commun Res Inst, Lab Nanoelect & Spintron, Sendai, Miyagi 9808577, Japan

来源：

ANALYTICAL SCIENCES | 2012年 / 28卷 / 11期

基金：

日本学术振兴会;

关键词：

POTASSIUM CHANNEL; MEMBRANES; HERG; RECONSTITUTION; RECTIFIER; PERMEATION; ELECTRODES; MONOLAYERS; DESIGN; CHIP;

D O I：

10.2116/analsci.28.1049

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this review, we will discuss our recent approaches for the formation of mechanically stable bilayer lipid membranes (BLMs) by combining with BLM formation and micro- and nano-fabrication techniques. BLMs were prepared across a microaperture fabricated in silicon (Si) chips or nanoporous alumina films using a minimized amount of organic solvent. Although BLMs spanned over the porous alumina film showed better electrical properties, such as background current noise and current transient, BLMs suspended in a thin Si3N4 septum showed a much superior BLM stability. The BLMs showed tolerance to a high voltage of +/- 1 V, a membrane lifetime of >40 h, and tolerance to repetitive solution exchanges. Application to a drug screening system has been examined by using the human ether-a-go-go-related gene (hERG) potassium channel as an illustrative example. The potentiality of the present system as a platform of the high-throughput analysis for ion-channel protein is also discussed.

引用

页码：1049 / 1057

页数：9

共 7 条

[1] Stable Lipid Bilayers Based on Micro- and Nano-Fabrication as a Platform for Recording Ion-Channel Activities
Ayumi Hirano-Iwata
Azusa Oshima
Hideki Mozumi
Yasuo Kimura
Michio Niwano
[J]. Analytical Sciences, 2012, 28 : 1049 - 1057
[2] Stable lipid bilayers based on micro- and nano-fabrication
Hirano-Iwata, Ayumi
Oshima, Azusa
Nasu, Tomohiro
Taira, Tasuku
Kimura, Yasuo
Niwano, Michio
[J]. SUPRAMOLECULAR CHEMISTRY, 2010, 22 (7-8) : 406 - 412
[3] Micro- and Nano-Technologies for Lipid Bilayer-Based Ion-Channel Functional Assays
Hirano-Iwata, Ayumi
Ishinari, Yutaka
Yamamoto, Hideaki
Niwano, Michio
[J]. CHEMISTRY-AN ASIAN JOURNAL, 2015, 10 (06) : 1266 - 1274
[4] Micro- and nano-fabrication of polymer based microlluidic platforms for BioMEMS applications
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[J]. BIOMEMS AND BIONANOTECHNOLOGY, 2002, 729 : 17 - 27
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[J]. 2011 34TH INTERNATIONAL SPRING SEMINAR ON ELECTRONICS TECHNOLOGY (ISSE 2011) - NEW TRENDS IN MICRO/NANOTECHNOLOGY, 2011, : 424 - 429
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[J]. BIOSENSORS & BIOELECTRONICS, 2009, 24 (07): : 1843 - 1849
[7] Micro-patterning of ionic reservoirs within a double bilayer lipid membrane to fabricate a 2D array of ion-channel switch based electrochemical biosensors
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Sapuri, A
Swanson, B
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[J]. NSTI NANOTECH 2004, VOL 1, TECHNICAL PROCEEDINGS, 2004, : 221 - 223

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