Development of an immunobinding dot-blot assay as an alternative for the serodiagnosis of human cysticercosis

被引：6
|
作者：
Hernandez-Cruz, E. [1 ]
Gonzalez-Cabriales, J. J. [1 ]
Ordaz-Pichardo, C. [1 ]
de la Cruz-Hernandez, N. I. [2 ]
Flores-Gutierrez, G. H. [1 ]
机构：
[1] Univ Autonoma Tamaulipas, Unidad Acad Multidisciplinaria Reynosa Aztlan, Reynosa, Tamaulipas, Mexico
[2] Univ Autonoma Tamaulipas, Fac Med Vet & Zootecnia Dr Norberto Trevino Zapat, Victoria, Tamaulipas, Mexico
来源：
JOURNAL OF HELMINTHOLOGY | 2009年 / 83卷 / 04期
关键词：
CEREBROSPINAL-FLUID; TAENIA-SOLIUM; MONOCLONAL-ANTIBODIES; MYCOPLASMA-BOVIS; NEUROCYSTICERCOSIS; ELISA; CELLULOSAE; DIAGNOSIS; ANTIGEN; MEXICO;
D O I：
10.1017/S0022149X09270866
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
An immunobinding dot-blot assay (DBA) was developed on nitrocellulose paper for the serodiagnosis of human cysticercosis, using Cysticercus cellulosae as antigen. The DBA had an immunological sensitivity of 0.08 mg of antigen protein/ml; however, it showed cross-reactions with antigens of adult Taenia solium and Echinococcus granulosus, but not with Toxoplasma gondii and Entamoeba histolytica antigens. An enzyme-linked immunosorbent assay (ELISA) was used as the gold standard for obtaining the diagnostic validity of the DBA, giving 84.61%, 100.00%, 100.00% and 97.98% for epidemiological sensitivity, epidemiological specificity and positive and negative predictive values, respectively. There were no statistical differences between the two tests (P < 0.05, kappa = 0.907). This study showed that DBA is an alternative method for the serodiagnosis of human cysticercosis.
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收藏
页码：333 / 337
页数：5
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