Condensation of fluorescent nanoparticles using a DEP chip with a dot-electrode array

被引:8
|
作者
Chuang, Cheng-Hsin [1 ]
Huang, Yao-Wei
机构
[1] So Taiwan Univ, Dept Mech Engn, Tainan 71005, Taiwan
关键词
Dielectrophoresis; Nanoparticle; Manipulation; BSA; IMMUNOASSAY; MANIPULATION; PARTICLES; CELL;
D O I
10.1016/j.mee.2012.04.015
中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
This paper develops a microfluidic chip for programmable condensation of fluorescent-labeled nanoparticles based on dielectrophoretic (DEP) force in order to enhance the strength of fluorescence density in a dot-electrode array. The structure of the DEP chip consists of a top electrode made of indium tin oxide (ITO) glass, a flow chamber formed by optically clear adhesive (OCA) tape, and a bottom 3 x 3 dot-electrode array. The vertically non-uniform electric field can trap nanoparticles on the bottom dot electrode using positive DEP force, and each dot electrode can be individually controlled by a computer-based program for programming manipulations. According to the experimental results, the fluorescence intensity was enhanced about three times when DEP force was applied to condense fluorescent-labeled nanoparticles on the dot-electrode surface. In addition, fluorescence intensity depends on the applied voltage as well as the applied time; an optimal operation parameter of condensation of fluorescent-labeled aluminium oxide (Al2O3) nanoparticles has been proposed based on parametric studies and experimental observations. Consequently, the present study demonstrates that the condensation of nanoparticles on a dot-electrode array using vertical DEP force instead of planar case and programmable manipulation, provides great potential in the application of multi-antibody arrays in a single chip for immunoassays. (c) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:317 / 323
页数:7
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