Selection and characterization of single-chain recombinant antibodies against spring viraemia of carp virus from mouse phage display library

被引：6
|
作者：
Liu, Hong [1 ,3 ]
Zheng, Xiaocong [1 ]
Zhang, Feng [2 ]
Yu, Li [1 ]
Zhang, Xiaohua [2 ]
Dai, Heping [2 ]
Hua, Qunyi [1 ]
Shi, Xiujie [1 ]
Lan, Wensheng [1 ]
Jia, Peng [1 ]
Yuan, Li [2 ]
Liu, Hong [1 ,3 ]
机构：
[1] Shenzhen Exit & Entry Inspect & Quarantine Bur, Key Lab Aquat Anim Dis, Shenzhen 518001, Peoples R China
[2] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
[3] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
来源：
JOURNAL OF VIROLOGICAL METHODS | 2013年 / 194卷 / 1-2期
关键词：
Phage display; Single-chain fragment variable; Spring viraemia of carp virus; MEDIATED ISOTHERMAL AMPLIFICATION; CYPRINUS-CARPIO; COMMON CARP; TECHNOLOGY; SEQUENCE; SVCV;
D O I：
10.1016/j.jviromet.2013.08.017
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Antibody-displaying phage library was selected after three rounds of panning against spring viraemia of carp virus (SVCV) by phage display technology. Eight positive clones which could produce soluble single-chain fragment variable (scFv) antibody induced by isopropyl-beta-D-thiogalactopyranoside (IPTG) were obtained. Dot blot results showed that the eight scFv antibodies could recognize SVCV. The soluble scFv antibodies showed a molecular weight 29 kD by Western blot. All scFv antibodies could recognize SVCV proteins specifically without cross-reaction with other virus proteins by ELISA. Indirect immunofluorescence results showed that all of these scFv antibodies reacted positively with virus in the SVCV-infected cells. These scFv antibodies will be useful tools to establish immunological detection methods for SVCV. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码：178 / 184
页数：7
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