Chemical tweaking of a non-fluorescent GFP chromophore to a highly fluorescent coumarinic fluorophore: application towards photo-uncaging and stem cell imaging

被引：17

作者：

Chatterjee, Tanmay ^{[1
]}

Roy, Debjit ^{[1
]}

Das, Ananya ^{[1
]}

Ghosh, Anup ^{[1
]}

Bag, Partha Pratim ^{[1
]}

Mandal, Prasun K. ^{[1
]}

机构：

[1] Indian Inst Sci Educ & Res IISER Kolkata, Dept Chem Sci, Kolkata 741252, W Bengal, India

来源：

RSC ADVANCES | 2013年 / 3卷 / 46期

关键词：

INTRAMOLECULAR PROTON-TRANSFER; PROTEIN CHROMOPHORE; STATE DYNAMICS; PHOTOISOMERIZATION; RELAXATION; ANALOGS;

D O I：

10.1039/c3ra44034f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Three step chemical tweaking of nonfluorescent parent GFP chromophore has yielded a three hundred times as bright coumarinic fluorophore. A fluorogenic compound has been prepared which after photo-uncaging yields the same highly bright fluorophore that could be used for live stem cell imaging.

引用

页码：24021 / 24024

页数：4