Camouflaging endothelial cells: does it prolong graft survival?

被引:15
|
作者
Stuhlmeier, KM [1 ]
Lin, Y [1 ]
机构
[1] Harvard Univ, Sch Med, Immunobiol Res Ctr, Beth Israel Deaconess Med Ctr, Boston, MA 02215 USA
来源
关键词
endothelial cell; organ rejection; immunoglobulin binding; complement binding;
D O I
10.1016/S0304-4165(99)00065-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Camouflaging antigens on the surface of cells seems an appealing way to prevent activation of the immune system. We explored the possibility of preventing hyperacute rejection by chemically camouflaging endothelial cells (EC). In vitro as well as in vivo experiments were performed. First, the ability of mPEG coating to prevent antibody-antigen interactions was evaluated. Second, we tested the degree to which mPEG coating prevents activation of EC by stimuli such as TNF-alpha and LPS. Third, in vivo experiments were performed to test the ability of mPEG coating to prolong xenograft survival. We demonstrate that binding of several antibodies to EC or serum proteins can be inhibited by mPEG. Furthermore, binding of TNF-alpha as well as LPS to EC is blocked since mPEG treatment of EC inhibits the subsequent up-regulation of E-selectin by these stimuli. However, in vivo experiments revealed that currently this method alone is not sufficient to prevent hyperacute rejection. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:177 / 190
页数:14
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