Stable transformation of cultured cells of the liverwort Marchantia polymorpha by particle bombardment

被引：5
|
作者：
Irifune, K
Ono, K
Takahashi, M
Murakami, H
Morikawa, H
机构：
[1] HIROSHIMA UNIV,FAC SCI,GRAD DEPT GENE SCI,HIGASHIHIROSHIMA 739,JAPAN
[2] KUMAMOTO UNIV,FAC SCI,DEPT BIOL SCI,KUMAMOTO 860,JAPAN
来源：
TRANSGENIC RESEARCH | 1996年 / 5卷 / 05期
关键词：
cultured liverwort cells; hygromycin phosphotransferase (HPT); Marchantia polymorpha; particle bombardment; stable transformation;
D O I：
10.1007/BF01968943
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Suspension-cultured cells (A-18 line) of the liverwort Marchantia polymorpha were bombarded by a pneumatic particle gun with plasmid pCH harbouring the hygromycin phosphotransferase (HPT) gene (hpt) under the control of the cauliflower mosaic virus (CaMV) 35S promoter and the nopaline synthase polyadenylation region. Nine weeks after bombardment. 128 hygromycin-resistant calluses were obtained from an approximate total of 7 x 10(6) cells. Ten cell lines chosen randomly were analysed further. Southern blot analysis showed that all of the ten lines contain the hpl gene in the genome, demonstrating that these lines are transformants. An HPT enzyme activity assay confirmed the expression of the gene in all of the transformant lines.
引用
收藏
页码：337 / 341
页数：5
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