A Robotics-Based Behavioral Paradigm to Measure Anxiety-Related Responses in Zebrafish

被引：64
|
作者：
Cianca, Valentina [1 ]
Bartolini, Tiziana [1 ]
Porfiri, Maurizio [1 ]
Macri, Simone [2 ]
机构：
[1] NYU, Polytech Inst, Dept Mech & Aerosp Engn, Brooklyn, NY USA
[2] Ist Super Sanita, Dept Cell Biol & Neurosci, Sect Behav Neurosci, I-00161 Rome, Italy
来源：
PLOS ONE | 2013年 / 8卷 / 07期
基金：
美国国家科学基金会;
关键词：
LIGHT/DARK PREFERENCE TEST; DANIO-RERIO RESPONDS; ALCOHOL; MODEL; FISH; STIMULI; ETHANOL; SCOTOTAXIS; WITHDRAWAL; PHENOTYPES;
D O I：
10.1371/journal.pone.0069661
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Zebrafish are gaining momentum as a laboratory animal species for the study of anxiety-related disorders in translational research, whereby they serve a fundamental complement to laboratory rodents. Several anxiety-related behavioral paradigms, which rest upon the presentation of live predatorial stimuli, may yield inconsistent results due to fatigue, habituation, or idiosyncratic responses exhibited by the stimulus itself. To overcome these limitations, we designed and manufactured a fully controllable robot inspired by a natural aquatic predator (Indian leaf fish, Nandus nandus) of zebrafish. We report that this robot elicits aversive antipredatorial reactions in a preference test and that data obtained therein correlate with data observed in traditional anxiety- and fear-related tests (light/dark preference and shelter-seeking). Finally, ethanol administration (0.25; 0.50; 1.00%) exerts anxiolytic effects, thus supporting the view that robotic stimuli can be used in the analysis of anxiety-related behaviors in zebrafish.
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页数：11
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