Shoot Tip Transformation in Papaya (Carica papaya L.)

被引：0
|
作者：
Chandra, R. [1 ]
Mishra, M. [1 ]
Pati, R. [1 ]
Agarwal, S. [2 ]
Jain, R. K. [2 ]
机构：
[1] Cent Inst Subtrop Hort, Biotechnol Lab, Lucknow 227107, Uttar Pradesh, India
[2] Indian Agr Res Inst, New Delhi, India
来源：
II INTERNATIONAL SYMPOSIUM ON PAPAYA | 2010年 / 851卷
关键词：
Carica papaya; transformation; shoot tip; acetosyringone; spermidine; AGROBACTERIUM-MEDIATED TRANSFORMATION; EFFICIENT TRANSFORMATION; RINGSPOT VIRUS; REGENERATION; PLANTS; TISSUES; GENE;
D O I：
暂无
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
In vitro regeneration and transformation protocol has been developed in papaya across the globe using somatic embryo as explant. Transgenic papaya has been developed from hypocotyle or immature zygote. The transformation efficiency remains very low through embryo mediated transformation due to lack of synchronized maturity and subsequently poor germination. We report here for the first time transformation of young, in vitro grown shoot tips of papaya with dual gene (cp+rep) construct through Agrobacterium.
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页数：7
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