Mercury detection based on label-free and isothermal enzyme-free amplified fluorescence platform

被引：12

作者：

Meng, Fanbin ^{[1
]}

Xu, Hui ^{[1
]}

Yao, Xue ^{[1
]}

Qin, Xuan ^{[2
]}

Jiang, Tingting ^{[3
]}

Gao, Shanmin ^{[1
]}

Zhang, Yahui ^{[1
]}

Yang, Di ^{[1
]}

Liu, Xia ^{[1
]}

机构：

[1] Ludong Univ, Sch Chem & Mat Sci, Yantai 264025, Peoples R China

[2] Lanzhou Univ Technol, Sch Mat Sci & Engn, Lanzhou 730050, Peoples R China

[3] Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China

来源：

TALANTA | 2017年 / 162卷

基金：

中国国家自然科学基金;

关键词：

Isothermal enzyme-free amplification; Label-free fluorescence platform; Hg2+; UNMODIFIED GOLD NANOPARTICLES; HIGHLY SENSITIVE DETECTION; COLORIMETRIC DETECTION; GRAPHENE OXIDE; MOLECULAR BEACON; HG2+ DETECTION; DNA DETECTION; ION HG2+; ONE-STEP; AMPLIFICATION;

D O I：

10.1016/j.talanta.2016.10.001

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel and convenient biosensor for Mercury (II) detection was developed based on toehold-mediated strand displacement isothermal enzyme-free amplification (EFA) technology and label-free fluorescence platform using Sybr Green I (SG) and graphene oxide (GO). The method is highly sensitive and selective, and the logarithmically related Hg2+ linearity range is from 0.1 to 50 nM with a detection limit 0.091 nM. Moreover, our strategy is simple, enzyme-free, and inexpensive and can be applied to detect spiked Hg2+ in environmental water samples with good recovery and accuracy, which demonstrates that the biosensor has a good potential in the environment surveys in the future.

引用

页码：368 / 373

页数：6

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