New Polymorphic EST-SSR Markers in Sugarcane

被引：5
|
作者：
Silva, Dennis Crystian [1 ]
Paz de Souza, Marislane Carvalo [1 ]
Costa Duarte Filho, Luiz Sergio [1 ]
dos Santos, Joao Messias [2 ]
de Souza Barbosa, Geraldo Verissimo [2 ]
Almeida, Cicero [1 ]
机构：
[1] Univ Fed Alagoas, Lab Genet Resources, Arapiraca, AL, Brazil
[2] Univ Fed Alagoas, Ctr Agr Sci, PMGCA RIDESA UFAL, Maceio, AL, Brazil
来源：
SUGAR TECH | 2012年 / 14卷 / 04期
关键词：
Microsatellites; Plant breeding; Saccharum; PCR-BASED ISOLATION; MICROSATELLITE MARKERS; GENETIC DIVERSITY; SEQUENCE; SACCHARUM; GENOME; IDENTIFICATION; OPTIMIZATION; SIMILARITY; GERMPLASM;
D O I：
10.1007/s12355-012-0184-7
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
New polymorphic SSR markers in sugarcane has potential usefulness to studies genetic diversity, genetic mapping, DNA fingerprinting and determination offspring as true hybrids, selfing or contaminant in germplasm from breeding program. SSR markers can be obtained in several ways, and the search for SSR-EST sequences an alternative with significant results. The aim of this study was to develop highly polymorphic SSR markers for germplasm studies in sugarcane. Di- tri- and tetra-nucleotide sequences were mined in the TGI bank and 53 EST-SSR markers developed. These markers were analyzed in five genotypes and showed to be highly polymorphic, with the PIC ranging between 0.48 and 0.95 with a mean of 0.86. The discriminatory power ranged from 0.35 to 0.94 with a mean of 0.82 and the genetic similarity was high in the accessions evaluated.
引用
收藏
页码：357 / 363
页数：7
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