Effects of regulated deficit irrigation applied at different growth stages of greenhouse grown tomato on substrate moisture, yield, fruit quality, and physiological traits

Given a critical water scarcity in arid and semi-arid Tunisian areas and aiming to reduce irrigation water request, it is crucial to identify and apply the best water-saving practices in these irrigated areas. Tomato is a high-water-requiring vegetable crop, thus increasing the pressure on water resources and environment. Its sustainable cultivation in such alarming conditions requires an adaptation of on-farm irrigation water-saving strategies preserving also the crop yield and leading to a fruit quality improvement. This study aimed to explore the effects of the regulated deficit irrigation (RDI) as an irrigation water-saving strategy, on yield, fruit quality, and physiological behavior of greenhouse grown potted tomato crop (Solanum lycopersicum L.) during three identified growth stages. The managed water regimes were (i) full irrigation (FI) ensuring 100% of the estimated water crop requirements, (ii) RDI1-25 and RDI1-50 ensuring respectively 75% and 50% of FI water supplies during the vegetative crop stage (stage I), (iii) RDI2-25 and RDI2-50 ensuring respectively 75% and 50% of FI water supplies from first truss blossom until first harvest (stage II), and (iv) RDI3-25 and RDI3-50 ensuring respectively 75% and 50% of FI supplies during the rest of the harvest period (stage III). The results showed that the substrate moisture vary significantly with the water deficit regime applied under different growth stages, thus providing different levels of substrate water content. Under RDI2, the tomato yield was the highest when compared with FI, RDI1, and RDI3 regimes. Indeed, saving the water by 20% under RDI2-50 reduced only 3% of tomato yield. Deficit irrigation under ripening fruit and flowering stages, mainly with 50% of water supplies shortage, resulted in higher fruit skin color, firmness, and refractometry index (degrees Brix) when compared to FI and RDI1. Physiological traits measurements indicated that FI exhibited the highest leaf stomatal conductance (gs) and chlorophyll index (CI) values while RDI3 exhibited the lowest gs and CI values among all the RDI treatments. Results are valuable in considering gs and CI as an efficient indicators of tomato plant water status. The results are also an important contribution to identify the second tomato growth stage as the best period that tomato plant tolerate water shortage without significant yield decrease, as well a rather fruit quality improvement. These results help to reach the challenge "more crop per drop" and can contribute to water scarcity remediation.