Molecular identification studies on Oestrus ovis L. (Diptera: Oestridae) larvae infested sheep in jazan region, Saudi Arabia

被引：6
|
作者：
Bosly, A. Hanan [1 ]
机构：
[1] Jazan Univ, Fac Sci, Biol Dept, Jazan, Saudi Arabia
来源：
INDIAN JOURNAL OF ANIMAL RESEARCH | 2018年 / 52卷 / 01期
关键词：
mtCOI; Oestrus ovis; PCR; Phylogeny and Sequence; HORSE NASAL MYIASIS; OXIDASE-I GENE; RHINOESTRUS SPP; FLIES; COI; INSECTS; CHINA;
D O I：
10.18805/ijar.v0iOF.8487
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
Oestrus ovis L. (O. ovis) (Diptera: Oestridae) (Sheep Bot Fly) is ubiquitous in distribution. Myiasis causing larvae collected from Abu-Arish area (Eastern Jazan), Saudi Arabia were confirmed as O. ovis based on morphological traits. Polymerase chain reaction (PCR) studies targeting amplification of partial fragment (606 bp) of mitochondrial cytochrome oxidase subunitI (mtCOI) gene further confirmed the species. Phylogenetic analysis based on the partial (mtCOI) gene sequence revealed that the accession KU921431 showed 97% similarity based on nucleotide pairs of O. ovis accessions, retrieved from the GenBank. Thus, a method of molecular identification of O. ovis larvae was established as a credible substitution to morphological identification in Jazan region, Saudi Arabia.
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收藏
页码：105 / 110
页数：6
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