Temperature gradient-based high-cell density fed-batch fermentation for the production of pyruvate oxidase by recombinant E-coli

被引：5
|
作者：
Liang, Jianguang [1 ]
Zhao, Jie [2 ]
Wang, Zejian [2 ]
Wang, Yonghong [2 ]
机构：
[1] Changshu Inst Technol, Sch Biol & Food Engn, Changshu, Jiangsu, Peoples R China
[2] East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
来源：
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY | 2018年 / 48卷 / 02期
关键词：
Escherichia coli DH5; pSML PyOD; fed-batch fermentation; high cell density; pyruvate oxidase; recombinant E; coli; temperature downshift; STABILITY; PHOSPHATE; PLASMID; GROWTH; WATER;
D O I：
10.1080/10826068.2018.1425709
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Pyruvate oxidase (PyOD) is a very powerful enzyme for clinical diagnostic applications and environmental monitoring. Influences of temperature on cell growth, plasmid stability, and PyOD expression during the PyOD fermentation process by recombinant Escherichia coli were investigated. Based on the influences of temperature on the physiological metabolism, a novel high-cell density fed-batch cultivation with gradient temperature decrease strategy for effective PyOD production was achieved, under which the biomass (OD600) of recombinant E. coli could reach to 71 and the highest PyOD activity in broth could reach to 3,307 U/L in 26hr fermentation.
引用
收藏
页码：188 / 193
页数：6
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