The structure of the O-antigen polysaccharide from Escherichia coli 0138 has been determined. NMR spectroscopy, together with component and methylation analyses, of native and reduced polysaccharide ware the principal methods used. The sequence of the sugar residues could be determined by NOESY and heteronuclear multiple bond connectivity (HMBC) NMR experiments. It is concluded that the polysaccharide is composed of tetrasaccharide repeating units with the following structure: -->4)-alpha-D-GalpNAcA-(1-->3)-D-GlcpNAc-(1-->2)-alpha-L-Rhap-(1-->3)-alpha-L-Rhap(1-->.
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Univ Stockholm, Arrhenius Lab, Dept Organ Chem, S-10691 Stockholm, SwedenUniv Stockholm, Arrhenius Lab, Dept Organ Chem, S-10691 Stockholm, Sweden
Fontana, Carolina
Weintraub, Andrej
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Karolinska Univ Hosp, Div Clin Microbiol, Dept Lab Med, Karolinska Inst, S-14186 Huddinge, SwedenUniv Stockholm, Arrhenius Lab, Dept Organ Chem, S-10691 Stockholm, Sweden
Weintraub, Andrej
Widmalm, Goran
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Univ Stockholm, Arrhenius Lab, Dept Organ Chem, S-10691 Stockholm, SwedenUniv Stockholm, Arrhenius Lab, Dept Organ Chem, S-10691 Stockholm, Sweden
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Stockholm Univ, Dept Organ Chem, Arrhenius Lab, S-10691 Stockholm, SwedenStockholm Univ, Dept Organ Chem, Arrhenius Lab, S-10691 Stockholm, Sweden
Fontana, Carolina
Weintraub, Andrej
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Karolinska Univ Hosp, Karolinska Inst, Div Clin Microbiol, Dept Lab Med, S-14186 Stockholm, SwedenStockholm Univ, Dept Organ Chem, Arrhenius Lab, S-10691 Stockholm, Sweden
Weintraub, Andrej
Widmalm, Goran
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Stockholm Univ, Dept Organ Chem, Arrhenius Lab, S-10691 Stockholm, SwedenStockholm Univ, Dept Organ Chem, Arrhenius Lab, S-10691 Stockholm, Sweden