Structural studies of the O-antigen polysaccharide from Escherichia coli O138

被引:6
|
作者
Linnerborg, M
Weintraub, A
Widmalm, G
机构
[1] UNIV STOCKHOLM,ARRHENIUS LAB,DEPT ORGAN CHEM,S-10691 STOCKHOLM,SWEDEN
[2] HUDDINGE UNIV HOSP,KAROLINSKA INST,DEPT IMMUNOL MICROBIOL PATHOL & INFECT DIS,DIV CLIN BACTERIOL,S-14186 HUDDINGE,SWEDEN
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1997年 / 247卷 / 02期
关键词
Escherichia coli; lipopolysaccharide; O-antigen; structure; NMR;
D O I
10.1111/j.1432-1033.1997.00567.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The structure of the O-antigen polysaccharide from Escherichia coli 0138 has been determined. NMR spectroscopy, together with component and methylation analyses, of native and reduced polysaccharide ware the principal methods used. The sequence of the sugar residues could be determined by NOESY and heteronuclear multiple bond connectivity (HMBC) NMR experiments. It is concluded that the polysaccharide is composed of tetrasaccharide repeating units with the following structure: -->4)-alpha-D-GalpNAcA-(1-->3)-D-GlcpNAc-(1-->2)-alpha-L-Rhap-(1-->3)-alpha-L-Rhap(1-->.
引用
收藏
页码:567 / 571
页数:5
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